Structure of PDB 5dn9 Chain B

Receptor sequence

>5dn9B (length=361) Species: 5477 ([Candida] boidinii)

MKIVLVLYDAGKHAADEEKLYGCTENKLGIANWLKDQGHELITTSDKEGG
NSVLDQHIPDADIIITTPFHPAYITKERIDKAKKLKLVVVAGVGSDHIDL
DYINQTGKKISVLEVTGSNVVSVAEHVLMTMLVLVRNFVPAHEQIINHDW
EVAAIAKDAYDIEGKTIATIGAGRIGYRVLERLVPFNPKELLYYDYQALP
KDAEEKVGARRVENIEELVAQADIVTINAPLHAGTKGLINKELLSKFKKG
AWLVNTARGAICVAEDVAAALESGQLRGYGGDVWFPQPAPKDHPWRDMRN
KYGAGNAMTPHYSGTTLDAQTRYAEGTKNILESFFTGKFDYRPQDIILLN
GEYITKAYGKH

3D structure

• PDB: 5dn9 Structural and Kinetic Studies of Formate Dehydrogenase from Candida boidinii.
• Chain: B
• Resolution: 1.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N119 R258 D282 Q287 H311
• Catalytic site (residue number reindexed from 1): N119 R258 D282 Q287 H311
• Enzyme Commision number: 1.17.1.9: formate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	V93 N119 V123 R174 I175 Y194 D195 Y196 A229 P230 H232 T235 T256 A257 R258 D282 H311 S313 G314 A357	V93 N119 V123 R174 I175 Y194 D195 Y196 A229 P230 H232 T235 T256 A257 R258 D282 H311 S313 G314 A357	MOAD: Kd=92uM
BS02	AZI	B	P68 F69 V93 R258 H311	P68 F69 V93 R258 H311

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008863 formate dehydrogenase (NAD+) activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0051287 NAD binding

Biological Process

• GO:0019752 carboxylic acid metabolic process
• GO:0042183 formate catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5dn9, PDBe:5dn9, PDBj:5dn9
• PDBsum: 5dn9
• PubMed: 27100912
• UniProt: A0A0A1EQY0