Structure of PDB 5cvu Chain B

Receptor sequence

>5cvuB (length=360) Species: 36903 (Clarkia breweri)

IQIIPTHSSDEEANLFAMQLASAAVLPMALKAAIELDVLEIMAKSVPPSG
YISPAEIAAQLPTTNPEAPVMLDRVLRLLASYSVVTYTLRELPSGKVERL
YGLAPVCKFLTKNEDGVSLAPFLLLATDKVLLEPWFYLKDAILEGGIPFN
KAYGMNIWDYFGTDHRINKVFNKGMSSNSTITMKKILEMYNGFEGLTTIV
DVGGGTGAVASMIVAKYPSINAINFDLPHVIQDAPAFSGVEHLGGDMFDG
VPKGDAIFIKWICHDWSDEHCLKLLKNCYAALPDHGKVIVAEYILPPSPD
PSIATKVVIHTDALMLAYNPGGKERTEKEFQALAMASGFRGFKVASCAFN
TYVMEFLKTA

3D structure

• PDB: 5cvu Structure of sinapyl alcohol bound monolignol 4-O-methyltransferase at 1.60 Angstroms resolution
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H272 D273 E300 E332
• Catalytic site (residue number reindexed from 1): H264 D265 E292 E324
• Enzyme Commision number: 2.1.1.146: (iso)eugenol O-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	55B	B	A134 W166 W269 H272 D273 L322 M323 Y326 N327	A126 W158 W261 H264 D265 L314 M315 Y318 N319
BS02	SAH	B	W166 M183 S187 G211 D234 L235 D254 M255 K268 I270	W158 M175 S179 G203 D226 L227 D246 M247 K260 I262

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0008171 O-methyltransferase activity
• GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
• GO:0046983 protein dimerization activity
• GO:0050630 (iso)eugenol O-methyltransferase activity

Biological Process

• GO:0032259 methylation

External links

• PDB: RCSB:5cvu, PDBe:5cvu, PDBj:5cvu
• PDBsum: 5cvu
• UniProt: O04385|IEMT_CLABR (Iso)eugenol O-methyltransferase (Gene Name=IEMT1)