Structure of PDB 5csl Chain B

Receptor sequence

>5cslB (length=2072) Species: 559292 (Saccharomyces cerevisiae S288C)

PGHFIGLNTVDKLEESPLRDFVKSHGGHTVISKILIANNGIAAVKEIRSV
RKWAYETFGDDRTVQFVAMATPEDLEANAEYIRMADQYIEVPGGTNNNNY
ANVDLIVDIAERADVDAVWAGWGHASENPLLPEKLSQSKRKVIFIGPPGN
AMRSLGDKISSTIVAQSAKVPCIPWSGTGVDTVHVDTGLVSVDDDIYQKG
CCTSPEDGLQKAKRIGFPVMIKASEGGGGKGIRQVEREEDFIALYHQAAN
EIPGSPIFIMKLAGRARHLEVQLLADQYGTNISLFGRDCSVQRRHQKIIE
EAPVTIAKAETFHEMEKAAVRLGKLVGYVSAGTVEYLYSHDDGKFYFLEL
NPRLQVEHPTTEMVSGVNLPAAQLQIAMGIPMHRISDIRTLYGMNPHSAS
EIDFEFKTQDATKKQRRPIPKGHCTACRITSEDPNDGFKPSGGTLHELNF
RSSSNVWGYFSVGNNGNIHSFSDSQFGHIFAFGENRQASRKHMVVALKEL
SIRGDFRTTVEYLIKLLETEDFEDNTITTGWLDDLITHKMTAEKPDPTLA
VICGAATKAFLASEEARHKYIESLQKGQVLSKDLLQTMFPVDFIHEGKRY
KFTVAKSGNDRYTLFINGSKCDIILRQLSDGGLLIAIGGKSHTIYWKEEV
AATRLSVDSMTTLLEVENDPTQLRTPSPGKLVKFLVENGEHIIKGQPYAE
IPLVSQENGIVQLLKQPGSTIVAGDIMAIMTLALPFEGMLPDFGSPVIEG
TKPAYKFKSLVSTLENILKGYDNQVIMNASLQQLIEVLRNPKLPYSEWKL
HISALHSRLPAKLDEQMEELVARSLRRGAVFPARQLSKLIDMAVKNPEYN
PDKLLGAVVEPLADIAHKYSNGLEAHEHSIFVHFLEEYYEVEKLFNGPNV
REENIILKLRDENPKDLDKVALTVLSHSKVSAKNNLILAILKHYQPLCKL
SSKVSAIFSTPLQHIVELESKATAKVALQAREILIQGALPSVKERTEQIE
HILKSSVVKVAYGPDLNILKDLIDSNYVVFDVLLQFLTHQDPVVTAAAAQ
VYIRRAYRAYTIGDIRVHEPIVEWKFQLPNRAVSVSDLSPLREGILMAVD
HLDDVDEILSQSLEVLSNVANVCVASTEGFESEEEILVRLREILDLNKQE
LINASIRRITFMFGFKDGSYPKYYTFNGPNYNENETIRHIEPALAFQLEL
GRLSNFNIKPIFTDNRNIHVYEAVSKTSPLDKRFFTRGIIRTGHIRDDIS
IQEYLTSEANRLMSDILDNLEVTDTSNSDLNHIFINFIAVFDISPEDVEA
AFGGFLERFGKRLLRLRVSSAEIRIIIKDPQTGAPVPLRALINNVSGYVI
KTEMYTEVKNAKGEWVFKSLGKPGSMHLRPIATPYPVKEWLQPKRYKAHL
MGTTYVYDFPELFRQASSSQWKNFSADVKLTDDFFISNELIEDENGELTE
VEREPGANAIGMVAFKITVKTPEYPRGRQFVVVANDITFKIGSFGPQEDE
FFNKVTEYARKRGIPRIYLAANSGARIGMAEEIVPLFQVAWNDAANPDKG
FQYLYLTSEGMETLKKFDKENSVLTERTVINGEERFVIKTIIGSEDGLGV
ECLRGSGLIAGATSRAYHDIFTITLVTCRSVGIGAYLVRLGQRAIQVEGQ
PIILTGAPAINKMLGREVYTSNLQLGGTQIMYNNGVSHLTAVDDLAGVEK
IVEWMSYVPAKRNMPVPILETKDTWDRPVDFTPTNDETYDVRWMIEGRET
ESGFEYGLFDKGSFFETLSGWAKGVVVGRARLGGIPLGVIGVETRTVENL
IPADPANPNSAETLIQEPGQVWHPNSAFKTAQAINDFNNGEQLPMMILAN
WRGFSGGQRDMFNEVLKYGSFIVDALVDYKQPIIIYIPPTGELRGGSWVV
VDPTINADQMEMYADVNARAGVLEPQGMVGIKFRREKLLDTMNRLDDKYR
ELRSQLHQQISKQLADRERELLPIYGQISLQFADLHDRSSRMVAKGVISK
ELEWTEARRFFFWRLRRRLNEEYLIKRLSHASRLEKIARIRSWYPASVDH
EDDRQVATWIEENYKTLDDKLK

3D structure

• PDB: 5csl Crystal structure of the 500-kDa yeast acetyl-CoA carboxylase holoenzyme dimer.
• Chain: B
• Resolution: 3.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E365 E379 N381 E387 R458 K599 V820 H846 S848 A977
• Catalytic site (residue number reindexed from 1): E335 E349 N351 E357 R428 K569 V775 H801 S803 A932
• Enzyme Commision number: 6.3.4.14: biotin carboxylase.
  6.4.1.2: acetyl-CoA carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BTI	B	F1956 G1998	F1854 G1896
BS02	COA	B	I1593 S1625 A1627 R1628 I1629 R1731 I1735	I1491 S1523 A1525 R1526 I1527 R1629 I1633

Gene Ontology

Molecular Function

• GO:0003989 acetyl-CoA carboxylase activity
• GO:0004075 biotin carboxylase activity
• GO:0005524 ATP binding
• GO:0016743 carboxyl- or carbamoyltransferase activity
• GO:0016874 ligase activity
• GO:0042802 identical protein binding
• GO:0042803 protein homodimerization activity
• GO:0046872 metal ion binding
• GO:1905502 acetyl-CoA binding

Biological Process

• GO:0006085 acetyl-CoA biosynthetic process
• GO:0006606 protein import into nucleus
• GO:0006633 fatty acid biosynthetic process
• GO:0042759 long-chain fatty acid biosynthetic process
• GO:0044281 small molecule metabolic process
• GO:2001295 malonyl-CoA biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005829 cytosol
• GO:0009317 acetyl-CoA carboxylase complex
• GO:0016020 membrane

External links

• PDB: RCSB:5csl, PDBe:5csl, PDBj:5csl
• PDBsum: 5csl
• PubMed: 26458104
• UniProt: Q00955|ACAC_YEAST Acetyl-CoA carboxylase (Gene Name=ACC1)