Structure of PDB 5c8t Chain B

Receptor sequence

>5c8tB (length=514)

AENVTGLFKDCSKIITGLHPTQAPTHLSVDIKFKTEGLCVDIPGIPKDMT
YRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATRDA
VGTNLPLQLGFSTGVNLVAVPTGYVDTENNTEFTRVNAKPPPGDQFKHLI
PLMYKGLPWNVVRIKIVQMLSDTLKGLSDRVVFVLWAHGFELTSMKYFVK
IGPERTCCLCDKRATCFSTSSDTYACWNHSVGFDYVYNPFMIDVQQWGFT
GNLQSNHDQHCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWSVEYPIIG
DELRVNSACRKVQHMVVKSALLADKFPVLHDIGNPKAIKCVPQAEVEWKF
YDAQPCSDKAYKIEELFYSYATHHDKFTDGVCLFWNCNVDRYPANAIVCR
FDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFTNLKQLPFFYYSDS
PCEYVPLKSATCITRCNLGGAVCRHHANEYRQYLDAYNMMISAGFSLWIY
KQFDTYNLWNTFTR

3D structure

• PDB: 5c8t Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10 complex
• Chain: B
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.-
  2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C210 C226 H229	C210 C226 H229
BS02	ZN	B	H257 C261 H264	H257 C261 H264
BS03	ZN	B	C452 C477 C484 H487	C452 C466 C473 H476
BS04	MG	B	D90 E191	D90 E191
BS05	SAM	B	G333 D352 A353 L366 F367 Y368 W385 N386 C387	G333 D352 A353 L366 F367 Y368 W385 N386 C387

Gene Ontology

Molecular Function

• GO:0000175 3'-5'-RNA exonuclease activity
• GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
• GO:0004532 RNA exonuclease activity
• GO:0008168 methyltransferase activity

External links

• PDB: RCSB:5c8t, PDBe:5c8t, PDBj:5c8t
• PDBsum: 5c8t
• PubMed: 26159422
• UniProt: P0C6X7|R1AB_SARS Replicase polyprotein 1ab (Gene Name=rep)