Structure of PDB 5c5h Chain B

Receptor sequence
>5c5hB (length=448) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
HMIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGFAVQGV
VEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLT
LQFALVPDGENTFPALTSLHIQLVEGAHAATWQPTRLCSMTLTSGGLPKA
AVHTYQAHLASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWKWLYAGA
RMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSLKAVLLGGAAIP
VELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIV
NNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRL
DNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEYD
HESVDLSEWVKDKLARFQQPVRWLTLPPELKGIKISRQALKEWVQRQQ
3D structure
PDB5c5h Mechanism of MenE Inhibition by Acyl-Adenylate Analogues and Discovery of Novel Antibacterial Agents.
ChainB
Resolution2.401 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T142 S162 H186 G270 E273 F356 G361
Catalytic site (residue number reindexed from 1) T143 S161 H185 G269 E272 F355 G360
Enzyme Commision number 6.2.1.26: o-succinylbenzoate--CoA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B N61 P183 N62 P182
BS02 4YB B H186 S188 L244 G246 G247 A248 A249 G270 L271 T272 T277 D336 K437 H185 S187 L243 G245 G246 A247 A248 G269 L270 T271 T276 D335 K434 MOAD: Kd=394nM
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008756 o-succinylbenzoate-CoA ligase activity
GO:0016874 ligase activity
GO:0016877 ligase activity, forming carbon-sulfur bonds
GO:0042802 identical protein binding
Biological Process
GO:0009234 menaquinone biosynthetic process
Cellular Component
GO:0032991 protein-containing complex

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Molecular Function
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Cellular Component
External links
PDB RCSB:5c5h, PDBe:5c5h, PDBj:5c5h
PDBsum5c5h
PubMed26394156
UniProtP37353|MENE_ECOLI 2-succinylbenzoate--CoA ligase (Gene Name=menE)

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