Structure of PDB 5ax9 Chain B

Receptor sequence
>5ax9B (length=274) Species: 9606 (Homo sapiens) [Search protein sequence]
IDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDE
EEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGS
VTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLT
ENAEVKLVDFGVSAIGTPYWMAPEVIADFKSDLWSLGITAIEMAEGAPPL
CDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQL
MKHPFIRDQPNERQVRIQLKDHID
3D structure
PDB5ax9 TNIK inhibition abrogates colorectal cancer stemness
ChainB
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D153 K155 Q157 N158 D171 T191
Catalytic site (residue number reindexed from 1) D141 K143 Q145 N146 D159 T167
Enzyme Commision number 2.7.11.1: non-specific serine/threonine protein kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 4KT B V31 V39 A52 K54 M105 F107 C108 G111 D115 L160 V170 D171 V19 V27 A40 K42 M93 F95 C96 G99 D103 L148 V158 D159 MOAD: ic50=8nM
BindingDB: IC50=8.0nM
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

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Molecular Function
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Biological Process
External links
PDB RCSB:5ax9, PDBe:5ax9, PDBj:5ax9
PDBsum5ax9
PubMed27562646
UniProtQ9UKE5|TNIK_HUMAN TRAF2 and NCK-interacting protein kinase (Gene Name=TNIK)

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