Structure of PDB 5aor Chain B

Receptor sequence

>5aorB (length=992) Species: 7227 (Drosophila melanogaster)

SVIDRIQEQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDDY
KYTPVGPEHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLVR
QLFHLNVIEPFSGTLKKKKDEQLKPYPVKLSPNLINKIDEVIKGLDLPVV
NPRNIKIELGPPIPLIVSVIPWAPPQANWNTWHACSIDDLSMDYERSLRD
RRQNDNEYRQFLEFREKLPIAAMRSEILTAINDNPVVIIRGNTGCGKTTQ
IAQYILDDYICSGQGGYANIYVTQPRRISAISVAERVARERCEQLGDTVG
YSVRFESVFPRPYGAILFCTVGVLLRKLEAGLRGVSHIIVDEIHERDVNS
DFLLVILRDMVDTYPDLHVILMSATIDTTKFSKYFGICPVLEVPGRAFPV
QQFFLEDIIQMTDFVPSAESRRKRKEVEDEEQLLSEDKDEAEINYNKVCE
DKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGAILVFLPGWNLI
FALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTKIILS
TNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRK
GRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEMALTIKLLRLGS
IHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPLGRLLARLPIEP
RLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQRRLANHQKALSG
TKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMSTMNVIWDAKQQ
LLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNICVHK
EKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQL
SMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELAAKIGALKPALE
DLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFGLQR

3D structure

• PDB: 5aor Structure of the RNA Helicase Mle Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling.
• Chain: B
• Resolution: 2.08 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H196 A197 R442 R443 R470 T486 V489 R492 K589 V635 F637 G662 W663 N664 A668 H691 S692 Q693 T717 N718 I719 R739 Y752 R861 P863 F894 E896 S899 L900 Q953 Q966 K1033 T1034 T1058	H59 A60 R276 R277 R304 T320 V323 R326 K423 V469 F471 G496 W497 N498 A502 H525 S526 Q527 T551 N552 I553 R573 Y586 R695 P697 F728 E730 S733 L734 Q787 Q800 K867 T868 T892
BS02	ADP	B	G410 G412 K413 T414 T415 R452 T725 R771 V772	G244 G246 K247 T248 T249 R286 T559 R605 V606
BS03	ALF	B	T409 K413 E508 S723 R768 R771	T243 K247 E342 S557 R602 R605

Gene Ontology

Molecular Function

• GO:0001069 regulatory region RNA binding
• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0003682 chromatin binding
• GO:0003690 double-stranded DNA binding
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0003725 double-stranded RNA binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0033679 3'-5' DNA/RNA helicase activity
• GO:0034458 3'-5' RNA helicase activity
• GO:0043138 3'-5' DNA helicase activity
• GO:0106222 lncRNA binding

Biological Process

• GO:0007549 sex-chromosome dosage compensation
• GO:0008340 determination of adult lifespan
• GO:0009047 dosage compensation by hyperactivation of X chromosome
• GO:0010468 regulation of gene expression
• GO:0031453 positive regulation of heterochromatin formation
• GO:0032508 DNA duplex unwinding
• GO:0042714 dosage compensation complex assembly
• GO:0045433 male courtship behavior, veined wing generated song production
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0048675 axon extension
• GO:0050684 regulation of mRNA processing
• GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
• GO:2000765 regulation of cytoplasmic translation

Cellular Component

• GO:0000228 nuclear chromosome
• GO:0000785 chromatin
• GO:0000805 X chromosome
• GO:0005634 nucleus
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0005829 cytosol
• GO:0016456 X chromosome located dosage compensation complex, transcription activating
• GO:0072487 MSL complex
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5aor, PDBe:5aor, PDBj:5aor
• PDBsum: 5aor
• PubMed: 26545078
• UniProt: P24785|MLE_DROME Dosage compensation regulator mle (Gene Name=mle)