Structure of PDB 5ajv Chain B

Receptor sequence
>5ajvB (length=435) Species: 9606 (Homo sapiens) [Search protein sequence]
LELTQSRVQKIWVPVDHLPRSNSPTVIVMVGLPARGKTYISKKLTRYLNW
IGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVK
SYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVV
ASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLS
LIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNL
QGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEAL
RLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGE
SYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLK
CPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERS
3D structure
PDB5ajv Structure-Based Design of Potent and Selective Inhibitors of the Metabolic Kinase Pfkfb3.
ChainB
Resolution3.01 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.1.105: 6-phosphofructo-2-kinase.
3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 8R2 B R45 Y49 I50 V214 V217 G218 L238 M239 I241 R35 Y39 I40 V204 V207 G208 L228 M229 I231 PDBbind-CN: -logKd/Ki=6.24,IC50=0.575uM
BindingDB: IC50=>30000nM
BS02 FDP B R252 H253 E322 Y333 R347 K351 Y362 H387 Q388 R392 R242 H243 E312 Y323 R337 K341 Y352 H377 Q378 R382
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003873 6-phosphofructo-2-kinase activity
GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016787 hydrolase activity
Biological Process
GO:0006000 fructose metabolic process
GO:0006003 fructose 2,6-bisphosphate metabolic process
GO:0006096 glycolytic process
GO:0006915 apoptotic process
GO:0010001 glial cell differentiation
GO:0016310 phosphorylation
GO:0046835 carbohydrate phosphorylation
GO:0061744 motor behavior
Cellular Component
GO:0005654 nucleoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5ajv, PDBe:5ajv, PDBj:5ajv
PDBsum5ajv
PubMed25849762
UniProtQ16875|F263_HUMAN 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (Gene Name=PFKFB3)

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