Structure of PDB 5a0t Chain B

Receptor sequence

>5a0tB (length=451) Species: 100226 (Streptomyces coelicolor A3(2))

SHPHPELGRPPALPKGGLRVTPLGGLGEIGRNMTVFEYGGRLLIVDCGVL
FPEEEQPGIDLILPDFTSIRDRLDDIEGIVLTHGHEDHIGGVPFLLREKP
DIPLIGSKLTLALIEAKLQEHRIRPYTLEVAEGHRERVGPFDCEFVAVNH
SIPDALAVAIRTPAGMVVHTGDFKMDQLPLDGRLTDLHAFARLSEEGIDL
LLADSTNAEVPGFVPPERDISNVLRQVFANARKRIIVASFASHVHRIQQI
LDAAHEYGRRVAFVGRSMVRNMGIARDLGYLKVPPGLVVDVKTLDDLPDS
EVVLVCTGSQGEPMAALSRMANRDHQIRIVNGDTVILASSLIPGNENAVY
RVINGLTRWGANVVHKGNAKVHVSGHASAGELLYFYNICRPKNLMPVHGE
WRHLRANAELGALTGVPHDRIVIAEDGVVVDLVEGKAKITGKVQAGYVYV
D

3D structure

• PDB: 5a0t Linkage of Catalysis and 5' End Recognition in Ribonuclease Rnase J
• Chain: B
• Resolution: 2.283 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	I30 F52 H86 E87 D88 F241 A242 S243 R267 S268 T308 E313 A316 S341 I343 P344 H373 S375 H377 H399	I29 F51 H85 E86 D87 F240 A241 S242 R266 S267 T307 E312 A315 S340 I342 P343 H372 S374 H376 H398
BS02	ZN	B	D88 H89 D173 H399	D87 H88 D172 H398
BS03	ZN	B	H84 H86 H151 D173	H83 H85 H150 D172

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5a0t, PDBe:5a0t, PDBj:5a0t
• PDBsum: 5a0t
• PubMed: 26253740
• UniProt: O86842