Structure of PDB 5a0m Chain B

Receptor sequence

>5a0mB (length=227) Species: 4932 (Saccharomyces cerevisiae)

KNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRD
EGKTYCMQFEWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGA
QTFKHQAFNKLANLFIVNNKKTIPNNLVENYLTPMSLAYWFMDDGGKWDY
NKNSTNTSIVLNTQSFTFEEVEYLVKGLRNKFQLNCYVKINKNKPIIYID
SMSYLIFYNLIKPYLIPQMMYKLPNTI

3D structure

• PDB: 5a0m Structure of the I-Scei Nuclease Complexed with its DsDNA Target and Three Catalytic Metal Ions.
• Chain: B
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	D344 E361 W362 K363 N390 L392 N394 V396 W449 N452 K493	D43 E60 W61 K62 N89 L91 N93 V95 W148 N151 K192
BS02	dna	B	P314 N315 K320 K323 S381 H384 K386 R388 Q402 F404 K405 Q465 S466 K493 K495	P13 N14 K19 K22 S80 H83 K85 R87 Q101 F103 K104 Q164 S165 K192 K194
BS03	dna	B	D344 D444 D445 Y451 N463 N492	D43 D143 D144 Y150 N162 N191
BS04	dna	B	G313 N315 K317 G343 D344 Y346 R348 R350 E361	G12 N14 K16 G42 D43 Y45 R47 R49 E60
BS05	MN	B	G343 D445	G42 D144
BS06	MN	B	D344 D445	D43 D144
BS07	MN	B	D344 D444	D43 D143

Gene Ontology

Molecular Function

• GO:0004519 endonuclease activity

Biological Process

• GO:0006314 intron homing
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0005739 mitochondrion

External links

• PDB: RCSB:5a0m, PDBe:5a0m, PDBj:5a0m
• PDBsum: 5a0m
• PubMed: 27303901
• UniProt: P03882|SCE1_YEAST Intron-encoded endonuclease I-SceI (Gene Name=SCEI)