Structure of PDB 4xxv Chain B

Receptor sequence

>4xxvB (length=355) Species: 271848 (Burkholderia thailandensis E264)

MKIAVLPGDGIGPEIVNEAVKVLNALDEKFELEHAPVGGAGYEASGHPLP
DATLALAKEADAILFGAVGDWKYDSLERALRPEQAILGLRKHLELFANFR
PAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGPF
AGEREGFDTMRYSEPEVRRIAHVAFQAAQKRAKKLLSVDKSNVLETSQFW
RDVMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVTGNMFGDILSD
EASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILSA
AMLLRYSLNRAEQADRIERAVKTVLEQGYRTGDIATPGCRQVGTAAMGDA
VVAAL

3D structure

• PDB: 4xxv Crystal structure of 3-isopropylmalate dehydrogenase from Burkholderia thailandensis in complex with NAD
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y135 K190 D222 D246 D250
• Catalytic site (residue number reindexed from 1): Y135 K190 D222 D246 D250
• Enzyme Commision number: 1.1.1.85: 3-isopropylmalate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	I11 A67 V68 G69 E83 I259 H279 G280 S281 A282 D284 I285 A291 N292	I11 A67 V68 G69 E83 I259 H279 G280 S281 A282 D284 I285 A291 N292

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003862 3-isopropylmalate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0046872 metal ion binding
• GO:0051287 NAD binding

Biological Process

• GO:0009098 L-leucine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:4xxv, PDBe:4xxv, PDBj:4xxv
• PDBsum: 4xxv
• UniProt: Q2T7H6|LEU3_BURTA 3-isopropylmalate dehydrogenase (Gene Name=leuB)