Structure of PDB 4xww Chain B

Receptor sequence

>4xwwB (length=543) Species: 1299 (Deinococcus radiodurans)

APTLEVIPLGGMGEIGKNITVFRYGDEIVVVDGGLAFPKAHQMGIDLIVP
RIDYLLEHQDKIKGWILTHGHEDHIGGLPYIFARLPRVPVYGLPLTLALV
REKLSEFGLQDVDLREVTYGDEVRFGQSFVAEFFCMTHSIPDNAGYILKT
PVGDVLHTGDFKIDPDVGTGAGIVSDLERVEQAGKDGVLLLISDSTNAER
PGHTPSEAEIARNLEEIIKGCRGRVFLTTFASQVYRIQNILDLAHRQGRR
VVMEGRSMIKYAQAAQATGHMNPPEPFLTSEEVGELQDQQVLFVCTGSQG
QPMAVLGRLAFGTHAKIALRRGDTVILSSNPIPGNEDAVNLIVNRLYEIG
VDVVYPPTYRVHASGHASQEELATILNLTRPKFFLPWHGEPRHQINHAKL
AQTLPRPPKRTLIAKNGDIVNLGPDEFRVSGTVAAGAVYVDGLGVGDVND
DVLLDRVNLSQEGLLILTAVLHPTPHVEVVARGFARPNRDLELQIRRVAL
EAVEQGLREKKRLEDVRDDMYGAVRRFTRKATGRNPVLIPMIV

3D structure

• PDB: 4xww Structural insights into catalysis and dimerization enhanced exonuclease activity of RNase J
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	I30 F52 H86 E87 D88 F245 A246 S247 R271 S272 T311 Q316 M318 R323 I347 P348 S379 G380 H381	I15 F37 H71 E72 D73 F230 A231 S232 R256 S257 T296 Q301 M303 R308 I332 P333 S364 G365 H366
BS02	ZN	B	D88 H89 H403	D73 H74 H388
BS03	ZN	B	H84 H86 H153	H69 H71 H138
BS04	MN	B	G59 D456	G44 D441

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4xww, PDBe:4xww, PDBj:4xww
• PDBsum: 4xww
• PubMed: 25940620
• UniProt: H9CZL7