Structure of PDB 4x1y Chain B

Receptor sequence

>4x1yB (length=428) Species: 9940 (Ovis aries)

EIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYN
EATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWA
KGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLI
SKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSI
DNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRK
LAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAA
CDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTA
VCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEG
MDEMEFTEAESNMNDLVSEYQQYQDATA

3D structure

• PDB: 4x1y Discovery of cytotoxic dolastatin 10 analogues with N-terminal modifications.
• Chain: B
• Resolution: 3.19 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GDP	B	G10 Q11 C12 S140 G143 G144 T145 S178 N206 Y224 N228	G8 Q9 C10 S136 G139 G140 T141 S174 N202 Y220 N224
BS02	LOC	B	C241 L242 L248 K254 L255 N258 M259 T314 K352	C237 L238 L244 K250 L251 N254 M255 T310 K348
BS03	3WV	B	Q15 K176 V177 D179 T223 Y224 G225	Q13 K172 V173 D175 T219 Y220 G221

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005200 structural constituent of cytoskeleton
• GO:0005525 GTP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0000226 microtubule cytoskeleton organization
• GO:0000278 mitotic cell cycle
• GO:0007017 microtubule-based process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005874 microtubule

External links

• PDB: RCSB:4x1y, PDBe:4x1y, PDBj:4x1y
• PDBsum: 4x1y
• PubMed: 25431858
• UniProt: D0VWY9