Structure of PDB 4wyg Chain B

Receptor sequence

>4wygB (length=429) Species: 83332 (Mycobacterium tuberculosis H37Rv)

LTPEQIIAVDGAHLWHPYSSIGREAVSPVVAVAAHGAWLTLIRDGQPIEV
LDAMSSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKLL
VDITPAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGY
HGDTFLAMSICDPHGGMHSLWTDVLAAQVFAPQVPRDYDPAYSAAFEAQL
AQHAGELAAVVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIA
TGFGRTGALFAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISA
GAAGALMHGPTFMANPLACAVSVASVELLLGQDWRTRITELAAGLTAGLD
TARALPAVTDVRVCGAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVY
AMPPYICTPAEITQITSAMVEVARLVGSL

3D structure

• PDB: 4wyg Fragment-Based Exploration of Binding Site Flexibility in Mycobacterium tuberculosis BioA.
• Chain: B
• Resolution: 1.62 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y25 Y157 E220 D254 A257 K283 Y407
• Catalytic site (residue number reindexed from 1): Y18 Y150 E213 D247 A250 K276 Y400
• Enzyme Commision number: 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	B	P317 T318	P310 T311
BS02	PLP	B	G124 S125 Y157 H158 E220 D254 I256 K283	G117 S118 Y150 H151 E213 D247 I249 K276
BS03	3W1	B	Y25 W64 W65 Y157 F402	Y18 W57 W58 Y150 F395	MOAD: Kd=6.8uM
BS04	3W1	B	R193 V222 H232 D233 P234 H271 C371	R186 V215 H225 D226 P227 H264 C364	MOAD: Kd=6.8uM

Gene Ontology

Molecular Function

• GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0009102 biotin biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4wyg, PDBe:4wyg, PDBj:4wyg
• PDBsum: 4wyg
• PubMed: 26068403
• UniProt: P9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)