Structure of PDB 4wtv Chain B

Receptor sequence

>4wtvB (length=426) Species: 9606,10665

MNAFLDDPEFADIMLRAEQAIEVGIFPERISSGSYFVKDPKRKIIGVFKP
KSEEPYGQTKYNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLITKDEAEK
LFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFT
NSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYKNLG
RGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFNYLPPKIGS
FQLFVEGYKEAEYWLRKFEADPLPENIRKQFQSQFERLVILDYIIRNTDR
GNDNWLVRYEKFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPF
SEEIRNLILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQ
ILNLTQALRDGKSPFQLVQIPCVIVE

3D structure

• PDB: 4wtv The high-resolution crystal structure of phosphatidylinositol 4-kinase II beta and the crystal structure of phosphatidylinositol 4-kinase II alpha containing a nucleoside analogue provide a structural basis for isoform-specific inhibitor design.
• Chain: B
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E1010 D1019
• Catalytic site (residue number reindexed from 1): E71 D80
• Enzyme Commision number: 2.7.1.67: 1-phosphatidylinositol 4-kinase.
  3.2.1.17: lysozyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	B	R271 K272	R266 K267
BS02	ATP	B	V146 K148 F259 V260 I343 D344	V47 K49 F254 V255 I319 D320

Gene Ontology

Molecular Function

• GO:0003796 lysozyme activity
• GO:0004430 1-phosphatidylinositol 4-kinase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds

Biological Process

• GO:0009253 peptidoglycan catabolic process
• GO:0016998 cell wall macromolecule catabolic process
• GO:0031640 killing of cells of another organism
• GO:0042742 defense response to bacterium
• GO:0044659 viral release from host cell by cytolysis

Cellular Component

• GO:0030430 host cell cytoplasm

External links

• PDB: RCSB:4wtv, PDBe:4wtv, PDBj:4wtv
• PDBsum: 4wtv
• PubMed: 26143926
• UniProt: P00720|ENLYS_BPT4 Endolysin (Gene Name=E);
  Q8TCG2