Structure of PDB 4wo5 Chain B

Receptor sequence
>4wo5B (length=257) Species: 9606 (Homo sapiens) [Search protein sequence]
WEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFK
NEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHASETKFE
MKKLIDIARQTARGMDYLHAKSIIHRDLKSNNIFLHEDNTVKIGDFGLAT
GSILWMAPEVIRMQDSNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQ
IIEMVGRGSLSPDLSKVRSNCPKRMKRLMAECLKKKRDERPSFPRILAEI
EELAREL
3D structure
PDB4wo5 Crystal structure of a BRAF kinase domain monomer explains basis for allosteric regulation.
ChainB
Resolution2.83 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D576 K578 N580 N581 D594 S616
Catalytic site (residue number reindexed from 1) D127 K129 N131 N132 D145 S152
Enzyme Commision number 2.7.11.1: non-specific serine/threonine protein kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 324 B V471 A481 K483 L514 T529 Q530 W531 C532 F583 D594 F595 V22 A32 K34 L65 T80 Q81 W82 C83 F134 D145 F146 BindingDB: IC50=13nM,Ki=2.6nM,Kd=100nM
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

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Molecular Function
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Biological Process
External links
PDB RCSB:4wo5, PDBe:4wo5, PDBj:4wo5
PDBsum4wo5
PubMed25437913
UniProtP15056|BRAF_HUMAN Serine/threonine-protein kinase B-raf (Gene Name=BRAF)

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