Structure of PDB 4un5 Chain B

Receptor sequence

>4un5B (length=1310) Species: 1314 (Streptococcus pyogenes)

KYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLF
DSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRL
IYLALAHMIKFRGHFLIEGDLNPDVDKLFIQLVQTYNQLFEENPINASGV
DAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNF
DLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDIL
RVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSK
NGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFD
NGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLA
RGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEK
VLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTN
RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDF
LDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRY
TGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKE
DIQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVI
EMARKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGR
DMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNV
PSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQ
LVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDF
QFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDGKATAKYF
FYSNIMNFFKTEIKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIV
KKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLV
VAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLI
IKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHDNEQ
KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITG
LYETRIDLSQ

3D structure

• PDB: 4un5 Structural Basis of Pam-Dependent Target DNA Recognition by the Cas9 Endonuclease
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	K33 L35 G56 E57 T58 A59 T62 R63 R66 R69 R70 R71 Y72 R74 R75 K76 N77 Y81 L101 E102 S104 F105 K111 R115 H116 V126 H129 T134 I135 F164 R165 G166 H167 Y325 H328 H329 K336 R340 F351 F352 Y359 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 K510 Y515 T519 N588 R661 K735 Q739 K742 T893 K1097 V1100 T1102 G1103 G1104 F1105 I1110 P1112 R1122 K1123 K1124 W1126 K1130 M1169 H1349 Q1350 S1351 Y1356	K30 L32 G53 E54 T55 A56 T59 R60 R63 R66 R67 R68 Y69 R71 R72 K73 N74 Y78 L98 E99 S101 F102 K108 R112 H113 V123 H126 T131 I132 F161 R162 G163 H164 Y319 H322 H323 K330 R334 F345 F346 Y353 I357 Q396 R397 T398 F399 N401 G402 F440 R441 I442 Y444 G447 P448 L449 A450 R451 N453 S454 F456 W458 R461 T466 I467 P469 K504 Y509 T513 N582 R655 K724 Q728 K731 T873 K1052 V1055 T1057 G1058 G1059 F1060 I1065 P1067 R1077 K1078 K1079 W1081 K1085 M1124 H1295 Q1296 S1297 Y1302
BS02	dna	B	K1107 E1108 S1109	K1062 E1063 S1064
BS03	dna	B	R1114 S1116 K1118 D1135 S1136 K1200 S1216 Q1221 R1333 R1335	R1069 S1071 K1073 D1090 S1091 K1155 S1171 Q1176 R1279 R1281
BS04	dna	B	Y450 N497 K526 E584 W659 N692 Q695 H698 A728 R925 Q926	Y444 N491 K520 E578 W653 N686 Q689 H692 A717 R905 Q906
BS05	MG	B	L623 T624 F626 Y656	L617 T618 F620 Y650
BS06	MG	B	I226 L229 E232	I220 L223 E226
BS07	MG	B	E584 R586	E578 R580

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:4un5, PDBe:4un5, PDBj:4un5
• PDBsum: 4un5
• PubMed: 25079318
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)