Structure of PDB 4tlz Chain B

Receptor sequence

>4tlzB (length=415) Species: 345341 (Kutzneria sp. 744)

PTHDVVGVGFGPANLSLAVALEESPAALTSAFFERRASISWHQGMLLPAA
KMQVSFLKDLATFRNPASRFSFVSFLHERGRLVRFANNHDFFPTRREFHD
YLEWAESKLAHEVSYDSEVTAIRPGPGRPVDSVLVDVSTPEATRTVEARN
IVISTGLVPRMPAGVQSDEFVWHSSRFLDHFRDRDPRSLRRVAVAGGGQS
AAEIVRFLHDNRPDTVVHAIMPSYGYVVADNTPFANQIFDPAAVDDYFDG
SKQAKDAFWRYHRNTNYSVVDDEVIRDLYRRGYDDEVAGAPRLNFVNLAH
VVGAKRIADDTRVTVYSMAREESYDLDVDVLVCATGYDPMDPGDLLGELA
EHCVQDAEGRWQVDRDYRMVTTPDLRCGIYLQGGTEHTHGLSSSLLSNLA
TRSGEIVSSIERRKS

3D structure

• PDB: 4tlz Crystallographic Evidence of Drastic Conformational Changes in the Active Site of a Flavin-Dependent N-Hydroxylase.
• Chain: B
• Resolution: 2.411 Å

Enzymatic activity

• Enzyme Commision number: 1.14.13.59: L-lysine N(6)-monooxygenase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	G18 G20 P21 A22 F42 E43 R44 W50 H51 R104 V128 T164 G165 L166 Y276 Y346 S403 L404	G9 G11 P12 A13 F33 E34 R35 W41 H42 R95 V119 T155 G156 L157 Y267 Y337 S394 L395
BS02	NAP	B	K60 Q62 R104 G205 G206 G207 Q208 S209 E212 P231 N275 S277 H309 V310 A343 T344 G345	K51 Q53 R95 G196 G197 G198 Q199 S200 E203 P222 N266 S268 H300 V301 A334 T335 G336
BS03	ORN	B	Q62 V63 K67 N245 F248 N275 S406	Q53 V54 K58 N236 F239 N266 S397

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004497 monooxygenase activity
• GO:0047091 L-lysine 6-monooxygenase (NADPH) activity

Biological Process

• GO:0009058 biosynthetic process

External links

• PDB: RCSB:4tlz, PDBe:4tlz, PDBj:4tlz
• PDBsum: 4tlz
• PubMed: 25184411
• UniProt: A8CF85