Structure of PDB 4rzb Chain B

Receptor sequence

>4rzbB (length=451) Species: 208964 (Pseudomonas aeruginosa PAO1)

SAIFAERALLPEGWARNVRFEISADGVLAEIRPDANADGAERLGGAVLPG
MPNLHSHAFQRAMAGLAEVAGNPNDSFWTWRELMYRMVARLSPEQIEVIA
CQLYIEMLKAGYTAVAEFHYVHHDLDGRSYADPAELSLRISRAASAAGIG
LTLLPVLYSHAGFGGQPASEGQRRFINGSEAYLELLQRLRAPLEAAGHSL
GLCFHSLRAVTPQQIATVLAAGHDDLPVHIHIAEQQKEVDDCQAWSGRRP
LQWLYENVAVDQRWCLVHATHADPAEVAAMARSGAVAGLCLSTEANLGDG
IFPATDFLAQGGRLGIGSDSHVSLSVVEELRWLEYGQRLRDRKRNRLYRD
DQPMIGRTLYDAALAGGAQALGQPIGSLAVGRRADLLVLDGNDPYLASAE
GDALLNRWLFAGGDRQVRDVMVAGRWVVRDGRHAGEERSARAFVQVLGEL
L

3D structure

• PDB: 4rzb Structure of N-Formimino-l-glutamate Iminohydrolase from Pseudomonas aeruginosa.
• Chain: B
• Resolution: 1.863 Å

Enzymatic activity

• Enzyme Commision number: 3.5.3.13: formimidoylglutamate deiminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NFQ	B	F78 R82 Y121 H206 R209 E235	F77 R81 Y120 H205 R208 E234	MOAD: Ki=30uM
BS02	ZN	B	H56 H58 H232 D320	H55 H57 H231 D319

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity
• GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
• GO:0019239 deaminase activity
• GO:0046872 metal ion binding
• GO:0050416 formimidoylglutamate deiminase activity

Biological Process

• GO:0006547 L-histidine metabolic process
• GO:0006548 L-histidine catabolic process
• GO:0019556 L-histidine catabolic process to glutamate and formamide
• GO:0019557 L-histidine catabolic process to glutamate and formate

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:4rzb, PDBe:4rzb, PDBj:4rzb
• PDBsum: 4rzb
• PubMed: 25559274
• UniProt: Q9HU77|HUTF_PSEAE Formimidoylglutamate deiminase (Gene Name=hutF)