Structure of PDB 4rve Chain B

Receptor sequence
>4rveB (length=240) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTY
NINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIY
3D structure
PDB4rve The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.
ChainB
Resolution3.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B T37 K92 T93 Y95 T106 K119 N120 S183 T186 N188 T36 K91 T92 Y94 T105 K118 N119 S182 T185 N187
BS02 dna B Q68 N70 S183 N185 T186 S223 Q67 N69 S182 N184 T185 S222
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:4rve, PDBe:4rve, PDBj:4rve
PDBsum4rve
PubMed8491171
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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