Structure of PDB 4rpu Chain B

Receptor sequence

>4rpuB (length=979) Species: 9606 (Homo sapiens)

AACERALQYKLGDKIHGFTVNQVTSVPELFLTAVKLTHDDTGARYLHLAR
EDTNNLFSVQFRTTPMDSTGVPHILQHTVLCGSQKYPCRDPFFKMLNRSL
STFMNAFTASDYTLYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEG
WRLEHENPSDPQTPLVFKGVVFNEMKGAFTDNERIFSQHLQNRLLPDHTY
SVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFPLEQHLKQIH
EEALSKFQKIEPSTVVPAQTPWDKPREFQITCGPDSFSKQTTVSVSFLLP
DITDTFEAFTLSLLSSLLTSGPNSPFYKALIESGLGTDFSPDVGYNGYTR
EAYFSVGLQGIVEKDIETVRSLIDRTIDEVVEKGFEDDRIEALLHKIEIQ
MKHQSTSFGLMLTSYIASCWNHDGDPVELLKLGNQLAKFRQCLQENPKFL
QEKVKQYFKNNQHKLTLSMRPDDKYHEKQAQVEATKLKQKVEALSPGDRQ
QIYEKGLELRSQQSKPQDASCLPALKVSDIEPTIPVTELDVVLTAGDIPV
QYCAQPTNGMVYFRAFSSLNTLPEELRPYVPLFCSVLTKLGCGLLDYREQ
AQQIELKTGGMSASPHVLPDDSHMDTYEQGVLFSSLCLDRNLPDMMQLWS
EIFNNPCFEEEEHFKVLVKMTAQELANGIPDSGHLYASIRAGRTLTPAGD
LQETFSGMDQVRLMKRIAEMTDIKPILRKLPRIKKHLLNGDNMRCSVNAT
PQQMPQTEKAVEDFLRSIGRSPVRTVEKPVIRKLVMEPTFKPWQMKTHFL
MPFPVNYVGECIRTVPYTDPDHASLKILARLMTAKFLHTEIREKGGAYGG
GAKLSHNGIFTLYSYRDPNTIETLQSFGKAVDWAKSGKFTQQDIDEAKLS
VFSTVDAPVAPSDKGMDHFLYGLSDEMKQAHREQLFAVSHDKLLAVSDRY
LGTGKSTHGLAILGPENPKIAKDPSWIIR

3D structure

• PDB: 4rpu Crystal Structure of Human Presequence Protease in Complex with Inhibitor MitoBloCK-60
• Chain: B
• Resolution: 2.265 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	H104 H108 E205	H73 H77 E174
BS02	3UE	B	G382 Y383 I432 Q435 M446 Y450 L467	G347 Y348 I397 Q400 M411 Y415 L432
BS03	3UE	B	I337 T340 F344 S347 Y380 X431 Y450 L464	I302 T305 F309 S312 Y345 X396 Y415 L429
BS04	CA	B	L629 E634	L594 E599

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008047 enzyme activator activity
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0006626 protein targeting to mitochondrion
• GO:0016485 protein processing

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix

External links

• PDB: RCSB:4rpu, PDBe:4rpu, PDBj:4rpu
• PDBsum: 4rpu
• UniProt: Q5JRX3|PREP_HUMAN Presequence protease, mitochondrial (Gene Name=PITRM1)