Structure of PDB 4rfr Chain B

Receptor sequence
>4rfrB (length=201) Species: 562 (Escherichia coli) [Search protein sequence]
PLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTN
CGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQ
PDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRND
PLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQAG
K
3D structure
PDB4rfr Rhein Inhibits AlkB Repair Enzymes and Sensitizes Cells to Methylated DNA Damage.
ChainB
Resolution1.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.14.11.33: DNA oxidative demethylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 RHN B N120 Y122 L128 D133 I143 S145 F154 W178 H187 I189 R204 N206 T208 N107 Y109 L115 D120 I130 S132 F141 W165 H174 I176 R191 N193 T195 MOAD: Kd=0.29uM
PDBbind-CN: -logKd/Ki=6.54,Kd=0.29uM
BS02 MN B H131 D133 H187 H118 D120 H174
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008198 ferrous iron binding
GO:0035515 oxidative RNA demethylase activity
GO:0035516 broad specificity oxidative DNA demethylase activity
GO:0046872 metal ion binding
GO:0051213 dioxygenase activity
Biological Process
GO:0006281 DNA repair
GO:0006307 DNA alkylation repair
GO:0035513 oxidative RNA demethylation
GO:0042245 RNA repair
GO:0070989 oxidative demethylation
GO:0072702 response to methyl methanesulfonate
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4rfr, PDBe:4rfr, PDBj:4rfr
PDBsum4rfr
PubMed27015802
UniProtP05050|ALKB_ECOLI Alpha-ketoglutarate-dependent dioxygenase AlkB (Gene Name=alkB)

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