Structure of PDB 4phc Chain B

Receptor sequence

>4phcB (length=441) Species: 9606 (Homo sapiens)

KFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKET
LMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHI
AKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSL
QIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNE
MVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDL
KLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQSVAAGG
RYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALKIRTTETQVLV
ASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLV
AIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQ

3D structure

• PDB: 4phc Comparison of histidine recognition in human and trypanosomatid histidyl-tRNA synthetases.
• Chain: B
• Resolution: 2.844 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.21: histidine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HIS	B	D130 T132 Q173 D177 R326 L328 Y330 Y331 G381 L382	D78 T80 Q121 D125 R274 L276 Y278 Y279 G320 L321

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004821 histidine-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0042802 identical protein binding
• GO:0042803 protein homodimerization activity

Biological Process

• GO:0006412 translation
• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006427 histidyl-tRNA aminoacylation
• GO:0032543 mitochondrial translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005829 cytosol

External links

• PDB: RCSB:4phc, PDBe:4phc, PDBj:4phc
• PDBsum: 4phc
• PubMed: 25151410
• UniProt: P12081|HARS1_HUMAN Histidine--tRNA ligase, cytoplasmic (Gene Name=HARS1)