Structure of PDB 4pfa Chain B

Receptor sequence

>4pfaB (length=416) Species: 83332 (Mycobacterium tuberculosis H37Rv)

TTATRLTGWGRTAPSVANVLRTPDAEMIVKAVARVAESGGGRGAIARGLG
RSYGDNAQNGGGLVIDMTPLNTIHSIDADTKLVDIDAGVNLDQLMKAALP
FGLWVPVLPGTRQVTVGGAIACDIHGKNHHSAGSFGNHVRSMDLLTADGE
IRHLTPTGEDAELFWATVGGNGLTGIIMRATIEMTPTSTAYFIADGDVTA
SLDETIALHSDGSEARYTYSSAWFDAISAPPKLGRAAVSRGRLATVEQLP
AKLRSEPLKFDGELWYRKSGTYRGKVQNLTQFYHPGFLQYQFVIPTEAVD
EFKKIIGVIQASGHYSFLNVFKLFGPRNQAPLSFPIPGWNICVDFPIKDG
LGKFVSELDRRVLEFGGRLYTAKDSRTTAETFHAMYPRVDEWISVRRKVD
PLRVFASDMARRLELL

3D structure

• PDB: 4pfa Crystal structure of M. tuberculosis in complex with BTO
• Chain: B
• Resolution: 2.56 Å

Enzymatic activity

• Enzyme Commision number: 1.1.98.3: decaprenylphospho-beta-D-ribofuranose 2-dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	W16 A53 R54 G55 L56 G57 R58 S59 Y60 N63 M74 P116 G117 T118 V121 T122 G125 A128 C129 I131 H132 N178 G179 I184 Y415	W9 A46 R47 G48 L49 G50 R51 S52 Y53 N56 M67 P109 G110 T111 V114 T115 G118 A121 C122 I124 H125 N171 G172 I177 Y370
BS02	N77	B	G117 G133 V365 C387	G110 G126 V320 C342

Gene Ontology

Molecular Function

• GO:0003885 D-arabinono-1,4-lactone oxidase activity
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0035884 arabinan biosynthetic process
• GO:0045227 capsule polysaccharide biosynthetic process
• GO:0046677 response to antibiotic
• GO:0070592 cell wall polysaccharide biosynthetic process
• GO:0071555 cell wall organization

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0042597 periplasmic space

External links

• PDB: RCSB:4pfa, PDBe:4pfa, PDBj:4pfa
• PDBsum: 4pfa
• UniProt: P9WJF1|DPRE1_MYCTU Decaprenylphosphoryl-beta-D-ribose oxidase (Gene Name=dprE1)