Structure of PDB 4o1p Chain B

Receptor sequence

>4o1pB (length=671) Species: 9823 (Sus scrofa)

SLEEMLTQAVQEADIEQVRQLLERGADANFQEEEWGWSPLHSAVQMDSED
LVALLLKHGADPCLRKRNGATPFIIAGITGNVRLLQLLLPNVEDVNECDV
NGFTAFMEAAVYGRVEALRFLYENGADVNMHRKTKQDQERIRKGGATALM
DAAEKGHVGVVTILLHAMKAEVDARDNMGRNALVYALLNPDDGKAKAITR
LLLDHGADVNVRGEGSKTPLILAVERKNLDLVQMLLEQEQIEVNDTDREG
KTALLLAVELRLEEIAKLLCHRGASTNCGDLVAIARRNYDSDLVKFLRLH
KNWKPQSSRWGEALKHLHRIWRPMIGKLKIFIDEEYKIADTAEGGIYLGL
YEDQEVAVKRFSEGSTRGQQEVSCLQSSRANDNVVTFYGSESDGSCLHVC
LALCEYTLQEHLANHRGDAVPNEEDESARNILSSLFKAIGELHRSGYSHQ
DLQPQNILIDSKNGTFLADFDKSIKWAEDPQKIKRDLEALGLLVLYVVKK
GDISFETLKNQSFEEVIQGSPDEETRDLIHHLFHPGEDRLSSLLAHPFFW
SWESRYRTLRDVGNESDIKTRNQNSRILQLLQPSTSFAQWTTKIDSFVME
EMNAYQDTLGDLLKFIRNLGEHINEQKNKKMKSIIGEPSQYFQEKFPDLV
MYVYTKLQNTEYMKHFPKTHN

3D structure

• PDB: 4o1p Dimeric structure of pseudokinase RNase L bound to 2-5A reveals a basis for interferon-induced antiviral activity.
• Chain: B
• Resolution: 2.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	25L	B	R307 R308 Y310 R353 F362	R286 R287 Y289 R322 F331
BS02	ANP	B	A370 T372 I377 A388 K390 C435 T438 Q486 L489 D500 D502	A339 T341 I346 A357 K359 C404 T407 Q455 L458 D469 D471
BS03	MG	B	D500 D502	D469 D471
BS04	MG	B	N487 D500	N456 D469
BS05	25L	B	W56 W58 Q66 K87 N89 N122 F124 E129 Y133 R153 K164	W35 W37 Q45 K66 N68 N101 F103 E108 Y112 R132 K143

Gene Ontology

Molecular Function

• GO:0004540 RNA nuclease activity
• GO:0004672 protein kinase activity
• GO:0005524 ATP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006397 mRNA processing
• GO:0006468 protein phosphorylation

External links

• PDB: RCSB:4o1p, PDBe:4o1p, PDBj:4o1p
• PDBsum: 4o1p
• PubMed: 24462203
• UniProt: A5H025