Structure of PDB 4nos Chain B

Receptor sequence
>4nosB (length=420) Species: 9606 (Homo sapiens) [Search protein sequence]
RHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDK
PTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLT
GDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVR
YSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPA
NVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAME
HPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRD
FCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVIHSFQKQNVT
IMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLN
YVLSPFYYYQVEAWKTHVWQ
3D structure
PDB4nos Structural characterization of nitric oxide synthase isoforms reveals striking active-site conservation.
ChainB
Resolution2.25 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C200 R203 W372 E377
Catalytic site (residue number reindexed from 1) C118 R121 W290 E295
Enzyme Commision number 1.14.13.39: nitric-oxide synthase (NADPH).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN B C110 C115 C28 C33
BS02 H2B B F476 H477 Q478 E479 F394 H395 Q396 E397
BS03 HEM B W194 C200 Q205 F369 G371 W372 E377 W463 Y489 Y491 W112 C118 Q123 F287 G289 W290 E295 W381 Y407 Y409
BS04 H4B B S118 M120 R381 I462 W463 S36 M38 R299 I380 W381
BS05 ITU B V352 F369 E377 V270 F287 E295 MOAD: Ki=19nM
BindingDB: IC50=160nM
Gene Ontology
Molecular Function
GO:0004517 nitric-oxide synthase activity
Biological Process
GO:0006809 nitric oxide biosynthetic process

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:4nos, PDBe:4nos, PDBj:4nos
PDBsum4nos
PubMed10074942
UniProtP35228|NOS2_HUMAN Nitric oxide synthase, inducible (Gene Name=NOS2)

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