Structure of PDB 4nme Chain B

Receptor sequence

>4nmeB (length=978) Species: 243231 (Geobacter sulfurreducens PCA)

NSELNTKIVNRGKEFFGSISGEKPSLFNKGAWMGKAMDWSMQNEQFKIQM
FRFVDVFPSLTTSKLLTEHIREYFGNEQDMPAFMAVLNKVLTSNIEEMAR
QFIVGETTKEAVKNLEKLRKDGFAAVVDVLGEATLSEEEAEVYTNTYLEL
LEALKKEQGSWKGLPGKGGDPGLDWGHAPKVNIAVKPTALFCLANPQDFE
GSVVAILDRMRRIFKKVMELNGFLCIDMESYRHKEIILEVFRRLKLEYRD
YPHLGIVLQAYLKDNDKDLDDLLAWAKEHKVQISVRLVKGAYWDYETVKA
KQNDWEVPVWTIKAESDAAYERQARKILENHQICHFACASHNIRTISAVM
EMARELNVPEDRYEFQVLYGMAEPVRKGILKVAGRIRLYAPYGNMVPGMG
YLVRRLLENTANESFLRQSFAEDAQIERLLEDPAVTVERERAARKGLGGL
PPFNNEAMVDFTRADHRAAFPKHIAQVRTQLGKTYPLFINGKEVRTNDLI
PTVNPNKPSEVLGQICQAGTTEVGDAIAAAKAAFPAWRDTDPRTRAEYLL
KAAQAARKRLFELSAWQVLEIGKQWDQAYADVTEAIDFLEYYAREMIRLG
QPQRVGHAPGELNHYFYEPKGVAAVIAPWNFPLAISMGMASAAIVTGNCV
VFKPSGITSIIGWHLVELFREAGLPEGVFNFTPGRGSVMGDYLVDHPDIS
LIAFTGSMETGLRIIERAAKVHPGQANVKKIISEMGGKNAIIIDDDADLD
EAVPHVLYSAFGFQGQKCSACSRVIVLDAVYDKFIERLVSMAKATKVGPS
EDPANYMGAVADDKAMKSIKEYAEIGKREGHVLYESPVPAGEGYFVPMTI
IGGIKPEHRIAQEEIFGPVLAVMRAKDFDQAIEWANSTQFALTGGIFSRS
PEHLAKARREFRVGNLYINRNNTGALVERQPFGGARMSGVGTKAGGPDYL
LHFMDPRVVTENTMRRGFAPIEEDDDWV

3D structure

• PDB: 4nme Structures of the PutA peripheral membrane flavoenzyme reveal a dynamic substrate-channeling tunnel and the quinone-binding site.
• Chain: B
• Resolution: 2.088 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N655 K678 E759 C793 E889 A969
• Catalytic site (residue number reindexed from 1): N630 K653 E734 C768 E864 A944
• Enzyme Commision number: 1.2.1.88: L-glutamate gamma-semialdehyde dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	P5F	B	K203 D244 M245 V274 Q276 R303 V305 K306 G307 A308 W310 W327 T328 I329 K330 S333 A356 S357 H358 N359 Q383 V384 L385 Y406 E425 E430 S431 F432	K186 D227 M228 V257 Q259 R286 V288 K289 G290 A291 W293 W310 T311 I312 K313 S316 A339 S340 H341 N342 Q366 V367 L368 Y389 E408 E413 S414 F415

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003700 DNA-binding transcription factor activity
• GO:0003842 1-pyrroline-5-carboxylate dehydrogenase activity
• GO:0004657 proline dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Biological Process

• GO:0006355 regulation of DNA-templated transcription
• GO:0006562 proline catabolic process
• GO:0010133 proline catabolic process to glutamate

Cellular Component

• GO:0005737 cytoplasm
• GO:0009898 cytoplasmic side of plasma membrane

External links

• PDB: RCSB:4nme, PDBe:4nme, PDBj:4nme
• PDBsum: 4nme
• PubMed: 24550478
• UniProt: Q746X3