Structure of PDB 4n76 Chain B

Receptor sequence

>4n76B (length=667) Species: 274 (Thermus thermophilus) [Search protein sequence]

HLGKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGRVYPLLAQVARRAG
GVTVRMGDGLASWSPPEVLVLEGTLARQTYAYRLYPKGRRPLDPKDPGER
SVLSALARRLLQERLRRLEGVWVEGLAVYRREHARGPGWRVLGGAVLDLW
VSDSGAFLLEVDPAYRILCEMSLEAWLAQGHPLPKRVRNAYDRRTWELLR
LEDPKELPLPGGLSLLDYHASKGRLQGREGGRVAWVARKPIPHLTGLLVP
VLTLEDLSLALSLPWEERRRRTREIASWIGRRLGLGTPEAVRAQAYRLSI
PKLMGRRAVSKPADALRVGFYRAQETALALLRLDGAQGWPEFLRRALLRA
FGASGASLRLHTLHAHPSQGLAFREALRKAKEEGVQAVLVLTPPMAWEDR
NRLKALLLREGLPSQILNVPLREEERHRWENALLGLLAKAGLQVVALSGA
YPAELAVGFDAGGRESFRFGGAACAVGGDGGHLLWTLPEAQAGERIPQEV
VWDLLEETLWAFRRKAGRLPSRVLLLRDGRVPQDEFALALEALAREGIAY
DLVSVRKSGGGRVYPVQGRLADGLYVPLEDKTFLLLTVHRDFRGTPRPLK
LVHEAGDTPLEALAHQIFHLTRLYPASGFAFPRLPAPLHLADRLVKEVGR
LGIRHLKEVDREKLFFV

3D structure

PDB

4n76 Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.

Chain

Resolution

2.89 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	B	A170 Y171 R172 I173 R192 R194 L265 S280 L281 R286 P412 M413 W415 K422 Q433 I434 N436 H445 R446 N449 K457 R580 R611 T613 P614 R615 A644 P650 R651 H657 R661 V685	A164 Y165 R166 I167 R186 R188 L252 S262 L263 R268 P394 M395 W397 K404 Q415 I416 N418 H427 R428 N431 K439 R562 R593 T595 P596 R597 A626 P632 R633 H639 R643 V667
BS02	dna	B	E268 S328 K329 H445 G481 K575 D590 V606 R608 F647 F649 K664	E255 S310 K311 H427 G463 K557 D572 V588 R590 F629 F631 K646
BS03	MN	B	D478 A479 D546	D460 A461 D528
BS04	MN	B	D478 D546 D660	D460 D528 D642
BS05	DT	B	Y197 L217 P218 Y226 H227 R232 P255 H256	Y191 L209 P210 Y218 H219 R224 P242 H243

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004520	DNA endonuclease activity
GO:0030145	manganese ion binding
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0044355	clearance of foreign intracellular DNA

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:4n76, PDBe:4n76, PDBj:4n76
PDBsum	4n76
PubMed	24374628
UniProt	Q746M7\|AGO_THET2 Protein argonaute (Gene Name=ago)

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