Structure of PDB 4mig Chain B

Receptor sequence

>4migB (length=574)

ADEPYDVFIAGSGPIGATFAKLCVDANLRVCMVEIGAADSFTSKPMKGQF
GPGQVPIPGYHKKNEIEYQKDIDRFVNVIKGALSTCSIPTSNNHIATLDP
SVVSNSLDKPFISLGKNPAQNPFVNLGAEAVTRGVGGMSTHWTCATPEFF
APADFNAPHRERPKLSTDAAEDARIWKDLYAQAKEIIGTSTTEFDHSIRH
NLVLRKYNDIFQKENVIREFSPLPLACHRLTDPDYVEWHATDRILEELFT
DPVKRGRFTLLTNHRCTKLVFKHYRPGEENEVDYALVEDLLPSVKKIYAR
SYVVACGAVATAQVLANSHIPPERDATIPTPLMPMLGKYITEQPMTFCQV
VLDSSLMEVVRNPPWPGLDWWKEKVARHVEAFPNDPIPIPFRDPEPQVTI
KFTEEHPWHVQIHRDAFSYGAVAENMDTRVIVDYRFFGYTEPQEANELVF
QQHYRDAYDMPQPTFKFTMSQDDRARARRMMDDMCNIALKIGGYLPGSEP
QFMTPGLALHLAGTTRCGLDTQKTVGNTHCKVHNFNNLYVGGNGVIETGF
AANPTLTSICYAIRASNDIIAKFG

3D structure

• PDB: 4mig Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly.
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 1.1.3.10: pyranose oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FDA	B	G22 P23 I24 E43 I44 T149 R150 G154 M155 H158 W159 T160 C161 A162 C283 C332 G333 A336 L552 N596 T598	G13 P14 I15 E34 I35 T132 R133 G137 M138 H141 W142 T143 C144 A145 C266 C306 G307 A310 L509 N553 T555
BS02	G3F	B	Q454 D458 F460 Y462 A551 H553 N596	Q411 D415 F417 Y419 A508 H510 N553

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
• GO:0050233 pyranose oxidase activity
• GO:0050660 flavin adenine dinucleotide binding

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:4mig, PDBe:4mig, PDBj:4mig
• PDBsum: 4mig
• PubMed: 24282677
• UniProt: Q6QWR1|P2OX_PHACH Pyranose 2-oxidase (Gene Name=p2ox)