Structure of PDB 4mfe Chain B

Receptor sequence

>4mfeB (length=594) Species: 347834 (Rhizobium etli CFN 42)

DRATKLLTYLADVTVNGHPEAKDRPKPLENAARPVVPYAGVKDGTKQLLD
TLGPKKFGEWMRNEKRVLLTDTTMRDGHQSLLATRMRTYDIARIAGTYSH
ALPNLLSLECWGGATFDVSMRFLTEDPWERLALIREGAPNLLLQMLLRGA
NGVGYTNYPDNVVKYFVRQAAKGGIDLFRVFDCLNWVENMRVSMDAIAEE
NKLCEAAICYTGDILNSARPKYDLKYYTNLAVELEKAGAHIIAVKDMAGL
LKPAAAKVLFKALREATGLPIHFHTHDTSGIAAATVLAAVEAGVDAVDAA
MDALSGNTSQPCLGSIVEALSGSERDPGLDPAWIRRISFYWEAVRNQYAA
FESDLKGPASEVYLHEMPGGQFTNLKEQARSLGLETRWHQVAQAYADANQ
MFGDIVKVTPSSKVVGDMALMMVSQDLTVADVVSPDREVSFPESVVSMLK
GDLGQPPSGWPEALQKKALKGEKPYTVRPGSLLKEADLDAERKVIEKKLE
REVSDFEFASYLMYPKVFTDFALASDTYGPVSVLPTPAYFYGLADGEELF
ADIEKGKTLVIVNQAVSATDSQGMVTVFFELNGQPRRIKVPDRA

3D structure

• PDB: 4mfe Insights into the carboxyltransferase reaction of pyruvate carboxylase from the structures of bound product and intermediate analogs.
• Chain: B
• Resolution: 2.61 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D549 D655 K718 H747 H749 T882
• Catalytic site (residue number reindexed from 1): D76 D182 K245 H274 H276 T409
• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D549 K718 H747 H749	D76 K245 H274 H276
BS02	3PY	B	R548 Q552 G586 L619 R621 K718 T882	R75 Q79 G113 L146 R148 K245 T409
BS03	BTN	B	Y479 D482 G487	Y9 D12 G17
BS04	MG	B	M534 R535 E537 D768	M61 R62 E64 D295

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis

External links

• PDB: RCSB:4mfe, PDBe:4mfe, PDBj:4mfe
• PDBsum: 4mfe
• PubMed: 24157795
• UniProt: Q2K340