Structure of PDB 4m6v Chain B

Receptor sequence

>4m6vB (length=593) Species: 347834 (Rhizobium etli CFN 42)

DRATKLLTYLADVTVNGHPEAKDRPKPLENARPVVPYAGVKDGTKQLLDT
LGPKKFGEWMRNEKRVLLTDTTMRDGHQSLLATRMRTYDIARIAGTYSHA
LPNLLSLECWGGATFDVSMRFLTEDPWERLALIREGAPNLLLQMLLRGAN
GVGYTNYPDNVVKYFVRQAAKGGIDLFRVFDCLNWVENMRVSMDAIAEEN
KLCEAAICYTGDILNSARPKYDLKYYTNLAVELEKAGAHIIAVKDMAGLL
KPAAAKVLFKALREATGLPIHFHTHDTSGIAAATVLAAVEAGVDAVDAAM
DALSGNTSQPCLGSIVEALSGSERDPGLDPAWIRRISFYWEAVRNQYAAF
ESDLKGPASEVYLHEMPGGQFTNLKEQARSLGLETRWHQVAQAYADANQM
FGDIVKVTPSSKVVGDMALMMVSQDLTVADVVSPDREVSFPESVVSMLKG
DLGQPPSGWPEALQKKALKGEKPYTVRPGSLLKEADLDAERKVIEKKLER
EVSDFEFASYLMYPKVFTDFALASDTYGPVSVLPTPAYFYGLADGEELFA
DIEKGKTLVIVNQAVSATDSQGMVTVFFELNGQPRRIKVPDRA

3D structure

• PDB: 4m6v The role of biotin and oxamate in the carboxyltransferase reaction of pyruvate carboxylase.
• Chain: B
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D549 D655 K718 H747 H749 T882
• Catalytic site (residue number reindexed from 1): D75 D181 K244 H273 H275 T408
• Enzyme Commision number: 6.4.1.1: pyruvate carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D549 K718 H747 H749	D75 K244 H273 H275
BS02	MG	B	M534 R535 E537 D768	M60 R61 E63 D294
BS03	PYR	B	R548 Q552 L619 R621 K718 T882	R74 Q78 L145 R147 K244 T408
BS04	BYT	B	Y479 D482 V483 G487 H488 R594 F595 T597 Y1001 R1066	Y9 D12 V13 G17 H18 R120 F121 T123 Y527 R592
BS05	BYT	B	R494 A556 F824 T846 E850	R24 A82 F350 T372 E376

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004736 pyruvate carboxylase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006090 pyruvate metabolic process
• GO:0006094 gluconeogenesis

External links

• PDB: RCSB:4m6v, PDBe:4m6v, PDBj:4m6v
• PDBsum: 4m6v
• PubMed: 25157442
• UniProt: Q2K340