Structure of PDB 4lem Chain B

Receptor sequence

>4lemB (length=543) Species: 83332 (Mycobacterium tuberculosis H37Rv)

HMDAITQVPVPANEPVHDYAPKSPERTRLRTELASLADHPIDLPHVIGGR
HRMGDGERIDVVQPHRHAARLGTLTNATHADAAAAVEAAMSAKSDWAALP
FDERAAVFLRAADLLAGPWREKIAAATMLGQSKSVYQAEIDAVCELIDFW
RFNVAFARQILEQQPISGPGEWNRIDYRPLDGFVYAITPFNFTSIAGNLP
TAPALMGNTVIWKPSITQTLAAYLTMQLLEAAGLPPGVINLVTGDGFAVS
DVALADPRLAGIHFTGSTATFGHLWQWVGTNIGRYHSYPRLVGETGGKDF
VVAHASARPDVLRTALIRGAFDYQGQKCSAVSRAFIAHSVWQRMGDELLA
KAAELRYGDITDLSNYGGALIDQRAFVKNVDAIERAKGAAAVTVAVGGEY
DDSEGYFVRPTVLLSDDPTDESFVIEYFGPLLSVHVYPDERYEQILDVID
TGSRYALTGAVIADDRQAVLTALDRLRFAAGNFYVNDKPTGAVVGRQPFG
GARGSDTNDKAGSPLNLLRWTSARSIKETFVAATDHIYPHMAV

3D structure

• PDB: 4lem Use of a "silver bullet" to resolve crystal lattice dislocation disorder: A cobalamin complex of Delta (1)-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis.
• Chain: B
• Resolution: 2.27 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N190 K212 E293 C327 E425 A510
• Catalytic site (residue number reindexed from 1): N191 K213 E294 C328 E426 A511
• Enzyme Commision number: 1.2.1.88: L-glutamate gamma-semialdehyde dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	B12	B	F246 T269 H272	F247 T270 H273

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003842 1-pyrroline-5-carboxylate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
• GO:0046872 metal ion binding

Biological Process

• GO:0006560 proline metabolic process
• GO:0010133 proline catabolic process to glutamate

Cellular Component

• GO:0009898 cytoplasmic side of plasma membrane

External links

• PDB: RCSB:4lem, PDBe:4lem, PDBj:4lem
• PDBsum: 4lem
• PubMed: 25557497
• UniProt: O50443