Structure of PDB 4l1f Chain B

Receptor sequence

>4l1fB (length=380) Species: 591001 (Acidaminococcus fermentans DSM 20731)

MDFNLTEDQQMIKDMAAEFAEKFLAPTVEERDKAHIWDRKLIDKMGEAGF
CGICFPEEYGGMGLDVLSYILAVEELSKVDDGTGITLSANVSLCATPIYM
FGTEEQKQKYLAPIAEGTHVGAFGLTEPSAGTDASAQQTTAVLKGDKYIL
NGSKIFITNGKEADTYVVFAMTDKSQGVHGISAFILEKGMPGFRFGKIED
KMGGHTSITAELIFEDCEVPKENLLGKEGEGFKIAMETLDGGRIGVAAQA
LGIAEGALAAAVKYSKEREQFGRSISKFQALQFMMADMATKIEAARYLVY
HAAMLKNEGKPYSEAAAMAKCFASDVAMEVTTDAVQIFGGYGYTVDYPAE
RYMRNAKITQIYEGTNQVMRIVTSRALLRD

3D structure

• PDB: 4l1f Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans.
• Chain: B
• Resolution: 1.79 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L125 T126 G242 E363 R375
• Catalytic site (residue number reindexed from 1): L125 T126 G242 E363 R375

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	R268 Q270 F271 I275 F278 L281 Q336 I337 G340	R268 Q270 F271 I275 F278 L281 Q336 I337 G340
BS02	FAD	B	F123 L125 T126 G131 T132 F156 I157 T158 I358 Y362 T365 Q367	F123 L125 T126 G131 T132 F156 I157 T158 I358 Y362 T365 Q367
BS03	COS	B	T132 H179 F232 M236 L239 D240 R243 Y362 E363 G364 R375	T132 H179 F232 M236 L239 D240 R243 Y362 E363 G364 R375

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003995 acyl-CoA dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
• GO:0050660 flavin adenine dinucleotide binding

External links

• PDB: RCSB:4l1f, PDBe:4l1f, PDBj:4l1f
• PDBsum: 4l1f
• PubMed: 24379410
• UniProt: D2RL84