Structure of PDB 4k2x Chain B

Receptor sequence
>4k2xB (length=490) Species: 1265868 (Streptomyces rimosus subsp. rimosus ATCC 10970) [Search protein sequence]
MRYDVVIAGAGPTGLMLACELRLAGARTLVLERLAEPVDFSKALGVHART
VELLDMRGLGEGFQAEAPKLRGGNFASLGVPLDFSSFDTRHPYALFVPQV
RTEELLTGRALELGAELRRGHAVTALEQDADGVTVSVTGPEGPYEVECAY
LVGCDGGGSTVRKLLGIDFPGQDPHMFAVIADARFREELPHGPYGVMRHD
LRAWFAAFPLEPDVYRATVAFFDRRAPVTEEDVRAALTEVAGSDFGMHDV
RWLSRLTDTSRQAERYRDGRVLLAGDACHIHLPAGGQGLNLGFQDAVNLG
WKLGATIAGTAPPELLDTYEAERRPIAAGVLRNTRAQAVLIDPDPRYEGL
RELMIELLHVPETNRYLAGLISALDVRYPMAGEHPLLGRRVPDLPLVTED
GTRQLSTYFHAARGVLLTLGCDQPLADEAAAWKDRVDLVAAEGVADPGSA
VDGLTALLVRPDGYICWTAAPETGTDGLTDALRTWFGPPA
3D structure
PDB4k2x Uncovering the Enzymes that Catalyze the Final Steps in Oxytetracycline Biosynthesis.
ChainB
Resolution2.55 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L44 L217 T225 P295
Catalytic site (residue number reindexed from 1) L44 L210 T218 P283
Enzyme Commision number 1.14.13.234: 12-dehydrotetracycline 5-monooxygenase.
1.14.13.38: anhydrotetracycline 6-monooxygenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD B G9 G11 P12 T13 L31 E32 R33 K42 A43 Q99 V123 D155 G156 T160 G287 D288 P295 G298 Q299 G300 L301 N302 G9 G11 P12 T13 L31 E32 R33 K42 A43 Q99 V123 D155 G156 T160 G275 D276 P283 G286 Q287 G288 L289 N290
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004497 monooxygenase activity
GO:0016491 oxidoreductase activity
GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0047670 anhydrotetracycline monooxygenase activity
GO:0071949 FAD binding
Biological Process
GO:0017000 antibiotic biosynthetic process

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Molecular Function
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Biological Process
External links
PDB RCSB:4k2x, PDBe:4k2x, PDBj:4k2x
PDBsum4k2x
PubMed23621493
UniProtL8EUQ6|OXYS_STRR1 12-dehydrotetracycline 5-monooxygenase/anhydrotetracycline 6-monooxygenase (Gene Name=oxyS)

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