Structure of PDB 4k2m Chain B

Receptor sequence

>4k2mB (length=439) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

MQKQVKISGKSKENMSLLKHLKGDVQGKELVIEDSIVNERWKQVLKEKID
IEHDLFNYQKNREISKVPFLPVDRLITNDEVEDILNTLTEVLPTGKFTSG
PYLEQFEKVLSTYLHKRYVIATSSGTDAIMIGLLALGLNPGDEVIMPANS
FSATENAVLASGGVPIYVDINPQTFCIDPDKIEEAITPYTKFILPVHLYG
KHSDMQHIRQIANRYKLKVIEDACQGIGLTDLGKYADITTLSFNPYKNFG
VCGKAGAIATDNEELAKKCIQFSYHGFEVNVKNKKVINFGFNSKMDNLQA
AIGLERMKYLSLNNFKRLFLADRYITQLAELQNKGYIELPELSEDHVWHL
FPIKVRTEDRADIMTKLNEDFGVQTDVYYPILSHMQKTPLVQDKYAGLQL
VHTEKAHSQVLHLPLYPSFTLEEQDRVMEGLFHVIKQEI

3D structure

• PDB: 4k2m The Structure of NtdA, a Sugar Aminotransferase Involved in the Kanosamine Biosynthetic Pathway in Bacillus subtilis, Reveals a New Subclass of Aminotransferases.
• Chain: B
• Resolution: 1.71 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): F151 D222 Q225 N244 K247 K284 K294
• Catalytic site (residue number reindexed from 1): F151 D222 Q225 N244 K247 K284 K294
• Enzyme Commision number: 2.6.1.104: 3-dehydro-glucose-6-phosphate--glutamate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	O1G	B	T98 S99 Y274 K282 N292	T98 S99 Y274 K282 N292
BS02	O1G	B	G125 T126 F151 A153 D222 C224 Q225 S242 N244 Y246 K247 Y379	G125 T126 F151 A153 D222 C224 Q225 S242 N244 Y246 K247 Y379

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0000271 polysaccharide biosynthetic process
• GO:0017000 antibiotic biosynthetic process

External links

• PDB: RCSB:4k2m, PDBe:4k2m, PDBj:4k2m
• PDBsum: 4k2m
• PubMed: 24097983
• UniProt: O07566|NTDA_BACSU 3-oxo-glucose-6-phosphate:glutamate aminotransferase (Gene Name=ntdA)