Structure of PDB 4j6c Chain B

Receptor sequence

>4j6cB (length=406) Species: 247156 (Nocardia farcinica IFM 10152)

PHSDTLTIDPMITDLAGETSRLRAAGPLTRIDLLGVPALAVTGHTLARQL
LTDTRLVKDINAWSLWQSGTVTRQWPLIGMIDVDRSMFTVDGPEHRRLRI
KTTQALTRRRLDALKPTIERYVAELLDDLERAGADGAVVDLKSVFAYPLP
MRVISALMGVPSEDQEQLLTWYKAFFSILTPQDERLRVIDEMHGYFTEMV
RRKTAEPGDDLTSALIYATDGETPLTEEEVIGNLQALVAAGHETTVSLIL
TAVRALLSHPEQLRLVRDGEIGWETAIEETLRWDGPVIHLLMRFATEDID
LGDAVIPRGEGVVMSYRAIGRDITVHGADADDFDITRATAARHISFGHGP
HICPGAALARLEAAIALPALFTRFPHLHPALPLDQIPNLPVLTQNDLSHF
PIHLGR

3D structure

• PDB: 4j6c Enzyme-substrate complex structures of CYP154C5 shed light on its mode of highly selective steroid hydroxylation.
• Chain: B
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S181 A244 E247 T248 T249 V291 C357 P358 G359 E366 Q398
• Catalytic site (residue number reindexed from 1): S177 A240 E243 T244 T245 V287 C353 P354 G355 E362 Q394

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	M91 H99 R103 L241 A244 G245 T248 T249 P290 L294 R297 Y320 S349 F350 H355 C357 P358 G359 A363	M87 H95 R99 L237 A240 G241 T244 T245 P286 L290 R293 Y316 S345 F346 H351 C353 P354 G355 A359
BS02	MG	B	H6 D8	H2 D4
BS03	STR	B	M84 F179 A240 A244 V291 L294	M80 F175 A236 A240 V287 L290

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding
• GO:0046872 metal ion binding

External links

• PDB: RCSB:4j6c, PDBe:4j6c, PDBj:4j6c
• PDBsum: 4j6c
• PubMed: 25372679
• UniProt: Q5YNS8