Structure of PDB 4iz6 Chain B

Receptor sequence
>4iz6B (length=612) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSY
RELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPV
LALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRV
VQLLNDSGEHNLQDAINHPAEDFTATPSPADEVAYFQLSGTGTPKLIPRT
HNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTV
VLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALIEGESRAQLASLK
LLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIH
TQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNAS
AFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLR
HPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKL
PDRVECVDSLPLTAVGKVDKKQLRQWLASRASAGRASIPASKAALREVIL
PLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTI
DAWWKLLSREVK
3D structure
PDB4iz6 Structure determination of the functional domain interaction of a chimeric nonribosomal peptide synthetase from a challenging crystal with noncrystallographic translational symmetry.
ChainB
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S190 S210 H234 A335 E336 N436 K441 K520
Catalytic site (residue number reindexed from 1) S189 S207 H231 A332 E333 N433 K438 K517
Enzyme Commision number 3.3.2.1: isochorismatase.
6.2.1.71: 2,3-dihydroxybenzoate--[aryl-carrier protein] ligase.
6.3.2.14: enterobactin synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PNS B I230 P231 H234 Y236 V279 G438 E440 I227 P228 H231 Y233 V276 G435 E437
BS02 1HZ B N235 Y236 S240 G309 A310 R311 V331 F332 G333 D415 K441 N232 Y233 S237 G306 A307 R308 V328 F329 G330 D412 K438
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008668 2,3-dihydroxybenzoate--[aryl-carrier protein] ligase
GO:0016746 acyltransferase activity
GO:0016874 ligase activity
GO:0016877 ligase activity, forming carbon-sulfur bonds
GO:0047527 2,3-dihydroxybenzoate-serine ligase activity
Biological Process
GO:0009239 enterobactin biosynthetic process
GO:0019290 siderophore biosynthetic process
Cellular Component
GO:0005829 cytosol
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4iz6, PDBe:4iz6, PDBj:4iz6
PDBsum4iz6
PubMed23897471
UniProtP0ADI4|ENTB_ECOLI Enterobactin synthase component B (Gene Name=entB);
P10378|ENTE_ECOLI Enterobactin synthase component E (Gene Name=entE)

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