Structure of PDB 4hxf Chain B

Receptor sequence

>4hxfB (length=613) Species: 70601 (Pyrococcus horikoshii OT3)

IEWDEKTFTKFAYLSDPRTRKNLVAYVLTKANLESNKYENTIVIENLEDG
SRKFIEDASMPRISPDGKKIAFMRFEKKTAQIWVADLKTLSAKKVLEAKN
IRSIEWNQDSRRLLAVGFKRREDEDFIFEDDVPAWFDNMGFFDGEKTTFW
VIDTEGEEVIEQFEKPRFSSGIWHGDSIVVSVPHRDVIPRYFKYWDIYLW
KDGEEEKLFEKVSFYAIDSDGERILLYGKPEKKYVSEHDKIYIYDGEVKG
ILDDIDREVAQAKIRNGKVYFTLFEEGSVNLYLWDGEVREIAKGKHWIMG
FDADERLIYLKETATRPAELYLWDGEERQLTDYNGLIFKKLKTFEPRHFR
FKSIDLELDGWYIKPEIKEGEKAPVIVFVHGGPKGMYGYYFKYEMQLMAS
KGYYIVYVNPRGSNGYSEDFALRVLERTGLEDFQDILNGIEEFLRLEPQA
DRERIGITGISYGGYMTNWALTQSDLFKAGISENGISYWLTSYAFSDIGL
WFDKEVIGDNPLENENYRKLSPLFYAKNVKAPLLLIHSLEDYRCPLDQSL
MFYHVLKDLGKEVYIAIFKKGAHGHSIRGSPRHRMKRYKLFMEFFERKLK
KYEEGFDVEKILK

3D structure

• PDB: 4hxf A Self-compartmentalizing Hexamer Serine Protease from Pyrococcus Horikoshii: SUBSTRATE SELECTION ACHIEVED THROUGH MULTIMERIZATION.
• Chain: B
• Resolution: 1.601 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H385 S466 Y467 D546 H580
• Catalytic site (residue number reindexed from 1): H380 S461 Y462 D541 H575
• Enzyme Commision number: 3.4.19.1: acylaminoacyl-peptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	Y3A	B	G386 G387 S466 F507 R548 C549 H578	G381 G382 S461 F502 R543 C544 H573
BS02	MG	B	D244 E263	D239 E258
BS03	MG	B	K99 E162	K94 E157

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008233 peptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:4hxf, PDBe:4hxf, PDBj:4hxf
• PDBsum: 4hxf
• PubMed: 23632025
• UniProt: O58323