Structure of PDB 4hvc Chain B

Receptor sequence

>4hvcB (length=483) Species: 9606 (Homo sapiens)

GLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFF
DAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAE
PIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRT
REFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKE
KFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGE
KQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGISEE
DKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLE
VGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASE
DLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTAMGAKSL
CIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

3D structure

• PDB: 4hvc ATP-directed capture of bioactive herbal-based medicine on human tRNA synthetase.
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.15: proline--tRNA ligase.
  6.1.1.17: glutamate--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	B	R1152 E1154 R1163 T1164 F1167 Q1237 T1240 T1276 R1278	R138 E140 R149 T150 F153 Q223 T226 T262 R264
BS02	HFG	B	H1093 F1097 E1100 V1101 P1120 T1121 E1123 R1152 F1216 H1242 W1273 G1274	H79 F83 E86 V87 P106 T107 E109 R138 F202 H228 W259 G260
BS03	ZN	B	C1448 C1453 C1495 C1497	C430 C435 C466 C468

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004827 proline-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006433 prolyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4hvc, PDBe:4hvc, PDBj:4hvc
• PDBsum: 4hvc
• PubMed: 23263184
• UniProt: P07814|SYEP_HUMAN Bifunctional glutamate/proline--tRNA ligase (Gene Name=EPRS1)