Structure of PDB 4h53 Chain B

Receptor sequence

>4h53B (length=388) Species: 382827 (Influenza A virus (A/RI/5+/1957(H2N2)))

VEYRNWSKPQCQITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPGKCYQ
FALGQGTTLDNKHSNDTVHDRIPHRTLLMNELGVPFHLGTRQVCIAWSSS
SCHDGKAWLHVCITGDDKNATASFIYDGRLVDSIGSWSQNILRTQESECV
CINGTCTVVMTDGSASGRADTRILFIEEGKIVHISPLSGSAQHIEECSCY
PRYPGVRCICRDNWKGSNRPVVDINMEDYSIDSSYVCSGLVGDTPRNDDS
SSNSNCRNPNNERGTQGVKGWAFDNGNDLWMGRTISKESRSGYETFKVIG
GWSTPNSKSQVNRQVIVDNNNWSGDSGIFSVEGKSCINRCFYVELIRGRP
QETRVWWTSNSIVVFCGTSGTYGTGSWPDGANINFMPI

3D structure

• PDB: 4h53 Influenza neuraminidase operates via a nucleophilic mechanism and can be targeted by covalent inhibitors
• Chain: B
• Resolution: 1.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D151 E277 R292 R371 D406
• Catalytic site (residue number reindexed from 1): D70 E196 R211 R290 D325
• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BMA	B	Q391 V392 R394	Q310 V311 R313
BS02	MAN	B	Q391 R394	Q310 R313
BS03	SIA	B	S367 S370 S372 N400 N401 N402 W403	S286 S289 S291 N319 N320 N321 W322
BS04	CA	B	D293 G297 D324 G345 Q347	D212 G216 D243 G264 Q266
BS05	SLB	B	R118 E119 D151 R152 W178 R224 A246 E276 E277 R292 R371	R37 E38 D70 R71 W97 R143 A165 E195 E196 R211 R290

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0046761 viral budding from plasma membrane

Cellular Component

• GO:0016020 membrane
• GO:0033644 host cell membrane
• GO:0055036 virion membrane

External links

• PDB: RCSB:4h53, PDBe:4h53, PDBj:4h53
• PDBsum: 4h53
• PubMed: 23422659
• UniProt: Q194T1