Structure of PDB 4h49 Chain B

Receptor sequence

>4h49B (length=159) Species: 9606 (Homo sapiens)

MGPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINT
GMADILVVFARGAHGDDHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWT
THSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADD
IRGIQSLYG

3D structure

• PDB: 4h49 Crystallization of bi-functional ligand protein complexes.
• Chain: B
• Resolution: 2.16 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H218 E219 H222 H228
• Catalytic site (residue number reindexed from 1): H114 E115 H118 H124
• Enzyme Commision number: 3.4.24.65: macrophage elastase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	L29	B	I180 L181 A182 L214 T215 H218 E219 H222 H228 P238 T239 Y240	I76 L77 A78 L110 T111 H114 E115 H118 H124 P134 T135 Y136
BS02	ZN	B	H218 H222 H228	H114 H118 H124
BS03	ZN	B	H168 D170 H183 H196	H64 D66 H79 H92
BS04	CA	B	D158 G190 G192 D194	D54 G86 G88 D90
BS05	CA	B	D124 E199 E201	D20 E95 E97
BS06	CA	B	D175 G176 G178 I180 D198 E201	D71 G72 G74 I76 D94 E97

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0031012 extracellular matrix

External links

• PDB: RCSB:4h49, PDBe:4h49, PDBj:4h49
• PDBsum: 4h49
• PubMed: 23567804
• UniProt: P39900|MMP12_HUMAN Macrophage metalloelastase (Gene Name=MMP12)