Structure of PDB 4gwm Chain B

Receptor sequence

>4gwmB (length=554) Species: 9606 (Homo sapiens)

PWENFDVDGGMDQDIFDINEGLGLDLFEGDIRLDRAQIRNSIIGEKYRWP
HTIPYVLEDSLEMNAKGVILNAFERYRLKTCIDFKPWAGETNYISVFKGS
GCWSSVGNRRVGKQELSIGANCDRIATVQHEFLHALGFWHEQSRSDRDDY
VRIMWDRILSGREHNFNTLNVPYDYTSVMHYSKTAFQNGTEPTIVTRISD
FEDVIGQRMDFSDSDLLKLNQLYNCSSSLSFMDSCSFELENVCGMIQNAD
WQRVSQVPRGPESDHSNGSGFFMHFDSSSVNVGATAVLESRTLYPKRGFQ
CLQFYLYNSGSESDQLNIYIREYSADNVDGNLTLVEEIKEIPTGSWQLYH
VTLKVTKKFRVVFEGRKGSGASLGGLSIDDINLSETRCPHHIWHIRNFTQ
FIGSPNGTLYSPPFYSSKGYAFQIYLNLAHVTNAGIYFHLISGANDDQLQ
WPCPWQQATMTLLDQNPDIRQRMSNQRSITTDPFMTTDNGNYFWDRPSKV
GTVALFSNGTQFRRGGGYGTSAFITHERLKSRDFIKGDDVYILLTVEDIS
HLNS

3D structure

• PDB: 4gwm Structural basis for the sheddase function of human meprin beta metalloproteinase at the plasma membrane
• Chain: B
• Resolution: 1.85 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H152 E153 H156 H162 Y211
• Catalytic site (residue number reindexed from 1): H130 E131 H134 H140 Y181
• Enzyme Commision number: 3.4.24.63: meprin B.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAN	B	Y454 N484	Y415 N445
BS02	MAN	B	S456 K457	S417 K418
BS03	MAN	B	N484 Q487	N445 Q448
BS04	ZN	B	D52 H152 H156 H162	D30 H130 H134 H140

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:4gwm, PDBe:4gwm, PDBj:4gwm
• PDBsum: 4gwm
• PubMed: 22988105
• UniProt: Q16820|MEP1B_HUMAN Meprin A subunit beta (Gene Name=MEP1B)