Structure of PDB 4g36 Chain B

Receptor sequence
>4g36B (length=543) Species: 7054 (Photinus pyralis) [Search protein sequence]
AKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVNITY
AEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVA
PANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMD
SKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSLPKG
VALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLI
CGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLS
NLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDD
KPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEA
TNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESI
LLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVT
TAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
3D structure
PDB4g36 Crystal structure of firefly luciferase in a second catalytic conformation supports a domain alternation mechanism.
ChainB
Resolution2.624 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S198 R218 H245 T343 E344 K443 Q448 K529
Catalytic site (residue number reindexed from 1) S195 R211 H238 T336 E337 K436 Q441 K522
Enzyme Commision number 1.13.12.7: firefly luciferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SLU B H245 F247 T251 G316 A317 P318 G339 Y340 G341 L342 T343 S347 A348 V362 D422 R437 K529 H238 F240 T244 G309 A310 P311 G332 Y333 G334 L335 T336 S340 A341 V355 D415 R430 K522
Gene Ontology
Molecular Function
GO:0004467 long-chain fatty acid-CoA ligase activity
GO:0004497 monooxygenase activity
GO:0005524 ATP binding
GO:0046872 metal ion binding
GO:0047077 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity
GO:0051087 protein-folding chaperone binding
Biological Process
GO:0001676 long-chain fatty acid metabolic process
GO:0008218 bioluminescence
GO:0046949 fatty-acyl-CoA biosynthetic process
Cellular Component
GO:0005777 peroxisome

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Cellular Component
External links
PDB RCSB:4g36, PDBe:4g36, PDBj:4g36
PDBsum4g36
PubMed22852753
UniProtP08659|LUCI_PHOPY Luciferin 4-monooxygenase

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