Structure of PDB 4g0u Chain B

Receptor sequence

>4g0uB (length=668) Species: 9606 (Homo sapiens) [Search protein sequence]

GIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPL
RGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKI
MIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITAKEAKEYFADM
ERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLTKHL
TYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVK
VAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTR
LHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIP
IIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYK
NFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPM
LNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQT
TLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGA
ESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEA
QGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKED
LAAFVEELDKVESQERED

3D structure

PDB

4g0u On the structural basis and design guidelines for type II topoisomerase-targeting anticancer drugs

Chain

Resolution

2.7 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	L507 N508 N520 H564 F669 I674 R677 K678 R820 Y821 I872 G873 T874 G875 W876 T1010 T1012 N1014 S1015	L55 N56 N68 H112 F172 I177 R180 K181 R311 Y312 I363 G364 T365 G366 W367 T501 T503 N505 S506
BS02	dna	B	E477 G504 K505 D561 R729 K739 Y773 H775 T783 N786 I872 W947	E25 G52 K53 D109 R220 K230 Y264 H266 T274 N277 I363 W438
BS03	dna	B	G453 D479 S480 R503 Q778	G1 D27 S28 R51 Q269
BS04	ASW	B	P455 R503 G504 E522	P3 R51 G52 E70	BindingDB: EC50=11000nM,IC50=2760nM
BS05	MG	B	D557 D559	D105 D107

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524	ATP binding

	Biological Process
GO:0006259	DNA metabolic process
GO:0006265	DNA topological change

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:4g0u, PDBe:4g0u, PDBj:4g0u
PDBsum	4g0u
PubMed	24038465
UniProt	Q02880\|TOP2B_HUMAN DNA topoisomerase 2-beta (Gene Name=TOP2B)

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