Structure of PDB 4foi Chain B

Receptor sequence

>4foiB (length=899) Species: 9606 (Homo sapiens) [Search protein sequence]

DDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLP
TFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTP
ENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDE
AILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMM
TCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFG
DDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAK
EGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSD
DDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVNGS
LYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAE
QHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSF
VRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIE
IMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGI
LITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTC
AYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDN
GCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKG
FLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDD
SILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLY
KLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTE

3D structure

PDB

4foi DETERMINATION of SITE AFFINITY CONSTANTS FOR PRODUCT INHIBITION OF WILD-TYPE AND MUTANT FORMS OF RECOMBINANT HUMAN HEXOKINASE TYPE I

Chain

Resolution

2.4 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R539 S603 T618 D657
Catalytic site (residue number reindexed from 1)	R524 S588 T603 D642
Enzyme Commision number	2.7.1.1: hexokinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	BGC	B	S155 F156 T172 K173 N208 D209 G233 N235 E260 E294	S140 F141 T157 K158 N193 D194 G218 N220 E245 E279
BS02	G16	B	D84 S88 R91 D209 T232 S415 S449	D69 S73 R76 D194 T217 S400 S434
BS03	BGC	B	S603 F604 T620 K621 N656 D657 N683 E708 E742	S588 F589 T605 K606 N641 D642 N668 E693 E727
BS04	G16	B	D532 T536 N537 D657 T680 D861 G862 T863 S897	D517 T521 N522 D642 T665 D846 G847 T848 S882

Gene Ontology

	Molecular Function
GO:0004340	glucokinase activity
GO:0004396	hexokinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0005536	D-glucose binding
GO:0008865	fructokinase activity
GO:0016301	kinase activity
GO:0016773	phosphotransferase activity, alcohol group as acceptor
GO:0019158	mannokinase activity
GO:0042834	peptidoglycan binding
GO:0047931	glucosamine kinase activity

	Biological Process
GO:0001678	intracellular glucose homeostasis
GO:0002720	positive regulation of cytokine production involved in immune response
GO:0005975	carbohydrate metabolic process
GO:0006002	fructose 6-phosphate metabolic process
GO:0006006	glucose metabolic process
GO:0006013	mannose metabolic process
GO:0006096	glycolytic process
GO:0006954	inflammatory response
GO:0009298	GDP-mannose biosynthetic process
GO:0016310	phosphorylation
GO:0019318	hexose metabolic process
GO:0032731	positive regulation of interleukin-1 beta production
GO:0045087	innate immune response
GO:0046835	carbohydrate phosphorylation
GO:0051156	glucose 6-phosphate metabolic process
GO:0061621	canonical glycolysis
GO:0061728	GDP-mannose biosynthetic process from mannose
GO:0072655	establishment of protein localization to mitochondrion
GO:0072656	maintenance of protein location in mitochondrion
GO:0141199	GDP-mannose biosynthetic process from glucose
GO:1901135	carbohydrate derivative metabolic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005741	mitochondrial outer membrane
GO:0005829	cytosol
GO:0045121	membrane raft

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:4foi, PDBe:4foi, PDBj:4foi
PDBsum	4foi
PubMed
UniProt	P19367\|HXK1_HUMAN Hexokinase-1 (Gene Name=HK1)

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