Structure of PDB 4ff3 Chain B

Receptor sequence

>4ff3B (length=1095) Species: 10752 (Enquatrovirus N4)

EELKEGIDAVYPSLVGTADSKAEGIKNYFKLSFTLPEEQKSRTVGSEAPL
KDVAQALSSRARYELFTEKETANPAFNGEVIKRYKELMEHGEGIADILRS
RLAKFLNTKDVGKRFAQGTEANRWVGGKLLNIVEQDGDTFKYNEQLLQTA
VLAGLQWRLTATSNTAIKDAKDVAAITGIDQALLPEGLVEQFDTGMTLTE
AVSSLAQKIESYWGLSRNPNAPLGYTKGIPTAMAAEILAAFVESTDVVEN
IVDMSEIDPDNKKTIGLYTITELDSFDPINSFPTAIEEAVLVNPTEKMFF
GDDIPPVANTQLRNPAVRNTPEQKAALKAEQATEFYVHTPMVQFYETLGK
DRILELMGAGTLNKELLNDNHAKSLEGKNRSVEDSYNQLFSVIEQVRAQS
EDISTVPIHYAYNMTRVGRMQMLGKYNPQSAKLVREAILPTKATLDLSNQ
NNEDFSAFQLGLAQALDIKVHTMTREVMSDELTKLLEGNLKPAIDMMVEF
NTTGSLPENAVDVLNTALGDRKSFVALMALMEYSRYLVAEDKSAFVTPLY
VEADGVTNGPINAMMLMTGGLFTPDWIRNIAKGGLFIGSPNKTMNEHRST
ADNNDLYQASTNALMESLGKLRSNYASNMPIQSQIDSLLSLMDLFLPDIN
LGENGALELKRGIAKNPLTITIYGSGARGIAGKLVSSVTDAIYERMSDVL
KARAKDPNISAAMAMFGKQAASEAHAEELLARFLKDMETLTSTVPVKRKG
VLELQSTGTGAKGKINPKTYTIKGEQLKALQENMLHFFVEPLRNGITQTV
GESLVYSTEQLQKATQIQSVVLEDMFKQRVQEKLAEKAKDPTWKKGDFLT
QKELNDIQASLNNLAPMIETGSQTFYIAGSENAEVANQVLATNLDDRMRV
PMSIYAPAQAGVAGIPFMTIGTGDGMMMQTLSTMKGAPKNTLKIFDGMNI
GLNDITDASRKANEAVYTSWQGNPIKNVYESYAKFMKNVDFSKLSPEALE
AIGKSALEYDQRENATVDDIANAASLIERNLRNIALGVDIRHKVLDKVNL
SIDQMAAVGAPYQNNGKIDLSNMTPEQQADELNKLFREELEARKQ

3D structure

• PDB: 4ff3 Watching the Bacteriophage N4 RNA Polymerase Transcription by Time-dependent Soak-trigger-freeze X-ray Crystallography.
• Chain: B
• Resolution: 1.997 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	K114 R119 W129 K176 D177 I181 T202 T204 E205 K267 K268 T269 G271 R318 R421 I675 Y678 S680 G681 K688 K849 K850 A888 D901 R902 R904 G916 V917 P921	K109 R114 W124 K171 D172 I176 T197 T199 E200 K262 K263 T264 G266 R313 R416 I670 Y673 S675 G676 K683 K844 K845 A883 D896 R897 R899 G911 V912 P916

External links

• PDB: RCSB:4ff3, PDBe:4ff3, PDBj:4ff3
• PDBsum: 4ff3
• PubMed: 23235152
• UniProt: Q859P9|RPOLV_BPN4 Virion DNA-directed RNA polymerase (Gene Name=50)