Structure of PDB 4eyh Chain B

Receptor sequence

>4eyhB (length=377) Species: 9606 (Homo sapiens)

SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGV
KKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGF
DENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLV
GSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPES
CQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGI
SVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELL
ASLLNRVCQDGRKPHTVRLIIRRGRESRQCPIPSHVIQKYDVMTPMVDIL
MKLFRNMVNVKMPFHLTLLSVCFCNLK

3D structure

• PDB: 4eyh Replication of a carcinogenic nitropyrene DNA lesion by human Y-family DNA polymerase.
• Chain: B
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R103 S276 P299 Q300 S301 F302 S303 E305 S307 R331 R347	R78 S251 P274 Q275 S276 F277 S278 E280 S282 R306 R322
BS02	dna	B	L123 E127 K207 P240 G241 G243 K245 T246 E358 S359 R360 Q361	L98 E102 K182 P215 G216 G218 K220 T221 E326 S327 R328 Q329
BS03	DCP	B	C37 Y39 Y68 R71 K77 D126 K214	C12 Y14 Y43 R46 K52 D101 K189
BS04	MG	B	D34 L35	D9 L10
BS05	MG	B	K237 I239 I242	K212 I214 I217
BS06	MG	B	D34 E127	D9 E102

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4eyh, PDBe:4eyh, PDBj:4eyh
• PDBsum: 4eyh
• PubMed: 23268450
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)