Structure of PDB 4ekk Chain B

Receptor sequence

>4ekkB (length=319) Species: 9606 (Homo sapiens)

RVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVA
HTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRER
VFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDF
GLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMM
CGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLG
GGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFT
AQMISERRPHFPQFDYSAS

3D structure

• PDB: 4ekk An ATP-Site On-Off Switch That Restricts Phosphatase Accessibility of Akt.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D274 K276 N279 D292 T312
• Catalytic site (residue number reindexed from 1): D131 K133 N136 D149 T169
• Enzyme Commision number: 2.7.11.1: non-specific serine/threonine protein kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	E234 F236 K276 E278 F309 C310 G311 T312 P313 E314 Y315 E341	E91 F93 K133 E135 F166 C167 G168 T169 P170 E171 Y172 E198
BS02	ANP	B	V164 A177 K179 M227 Y229 A230 E234 K276 M281 T291 D292 F438	V21 A34 K36 M84 Y86 A87 E91 K133 M138 T148 D149 F295
BS03	MN	B	N279 D292	N136 D149

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0004674 protein serine/threonine kinase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006468 protein phosphorylation

External links

• PDB: RCSB:4ekk, PDBe:4ekk, PDBj:4ekk
• PDBsum: 4ekk
• PubMed: 22569334
• UniProt: P31749|AKT1_HUMAN RAC-alpha serine/threonine-protein kinase (Gene Name=AKT1)