Structure of PDB 4dt2 Chain B

Receptor sequence

>4dt2B (length=426) Species: 3885 (Phaseolus vulgaris)

KNRDMPLDSDVFRVPPGYNAPQQVHITQGDLVGRAMIISWVTMDEPGSSA
VRYWSEKNGRKRIAKGKMSTYRFFNYSSGFIHHTTIRKLKYNTKYYYEVG
LRNTTRRFSFITPPQTGLDVPYTFGLIGDLGQSFDSNTTLSHYELSPKKG
QTVLFVGDLSYADRYPNHDNVRWDTWGRFTERSVAYQPWIWTAGNHEIEF
APEINETEPFKPFSYRYHVPYEASQSTSPFWYSIKRASAHIIVLSSYSAY
GRGTPQYTWLKKELRKVKRSETPWLIVLMHSPLYNSYNHHFMEGEAMRTK
FEAWFVKYKVDVVFAGHVHAYERSERVSNIAYKITNGLCTPVKDQSAPVY
ITIGDAGNYGVIDSNMIQPQPEYSAFREASFGHGMFDIKNRTHAHFSWNR
NQDGVAVEADSVWFFNRHWYPVDDST

3D structure

• PDB: 4dt2 Identification of purple acid phosphatase inhibitors by fragment-based screening: promising new leads for osteoporosis therapeutics.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D135 D164 Y167 N201 H202 H286 H295 H296 H323 H325
• Catalytic site (residue number reindexed from 1): D129 D158 Y161 N195 H196 H280 H289 H290 H317 H319
• Enzyme Commision number: 3.1.3.2: acid phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D164 N201 H286 H323	D158 N195 H280 H317
BS02	FE	B	D135 D164 Y167 H325	D129 D158 Y161 H319
BS03	0LV	B	N201 H202 H295 H296 H323 H325 Y365	N195 H196 H289 H290 H317 H319 Y359

Gene Ontology

Molecular Function

• GO:0003993 acid phosphatase activity
• GO:0008199 ferric iron binding
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:4dt2, PDBe:4dt2, PDBj:4dt2
• PDBsum: 4dt2
• PubMed: 22943065
• UniProt: P80366|PPAF_PHAVU Fe(3+)-Zn(2+) purple acid phosphatase