Structure of PDB 4cmq Chain B

Receptor sequence

>4cmqB (length=1168) Species: 301447 (Streptococcus pyogenes serotype M1)

KYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLF
DSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
HPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFR
GHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSAR
LSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQ
LSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVTEITKAP
LSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGG
ASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFNGSIPHQIHLG
ELHAILRRQEDFYPFLKDNREKIEKILTFRLPNEKVLPKHSLLYEYFTVY
NELTKVKYVTFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKFNASLGTY
HDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFD
DKVMKQLKRRRYTGWGRLSRKLINGIRDKGKTILDFLKSDGFANRNFMQL
IHDDSLTFKEDIQKAQVDSLHEHIANLAGSPAIKKGILQTVKVVDELVKV
MGRHKPENIVIEMAREKNSRERMKRIEEGIKELEHPVENTQLQNEKLYLY
YLQNGRDMYVDQELDINRLSDVDHIVPQSFLKDDSIDNKVLTRNRGKSDN
VPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKLSELDKAGFIKRQLVET
RQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYK
VREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAK
SYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVR
KVLSMPQVNIVKKTEVQTPTVAYSVLVVAKVKELLGITIMERSSFEKNPI
DFLEAKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKY
VNFLYLASHKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYN
KHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDA
TLIHQSITGLYETRIDLS

3D structure

• PDB: 4cmq Structures of Cas9 Endonucleases Reveal RNA- Mediated Conformational Activation
• Chain: B
• Resolution: 3.09 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	B	D10 E766 H983	D7 E666 H859
BS02	MN	B	D10 E762 E766	D7 E662 E666
BS03	MN	B	L380 T386	L364 T370
BS04	MN	B	H511 E516	H440 E445

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:4cmq, PDBe:4cmq, PDBj:4cmq
• PDBsum: 4cmq
• PubMed: 24505130
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)